Developing eight SNP-STR markers for DNA mixture detection
Developing eight SNP-STR markers for DNA mixture detection
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开发 8 个用于 DNA 混合物检测的 SNP-STR 标记
DOI：
10.1016/j.fsigss.2017.09.114
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发表时间：
2017-12
影响因子：
--
通讯作者：
Lin Zhang
中科院分区：
文献类型：
--
作者：
Yu Tan;Hui Wang;Tao Feng;Li Wang;Weibo Liang;Lin Zhang
SNP-STR标记是由STR标记和侧翼序列中的SNP构成的复合标记。SNP-STR标记的引入提高了STR标记的识别能力。近年来，有研究报道通过设计两个等位基因特异性引物，SNP-STR标记在检测不平衡DNA混合物方面表现良好。本研究选择了在中国汉族人群中具有高度多态性的STR标记。然后从SNP数据库中筛选出次要等位基因频率（MAF）大于0.10且位于STR标记重复序列250 bp以内的SNP标记。在此基础上，筛选出8个SNP-STR标记，采用扩增难治性突变系统（ARMS-PCR）方法进行等位基因特异性扩增。利用20个样本对中国汉族人群SNPs多态性进行了研究。8个位点中有6个位点具有较高的SNP多态性（> 0.20），表明该位点具有区分DNA混合物的潜力。大多数引物靶向超过100倍主要DNA的次要DNA。然而，使用这六个位点的辨别力不够高。因此，有必要进一步研究开发更多的SNP-STR标记。
SNP-STR marker is a compound marker constituted by a STR marker and a SNP in the flanking sequence. SNP-STR marker has been introduced to improve the discrimination power of STR markers in previous reports. Recently, some researchers have reported that SNP-STR markers performed well in detecting unbalanced DNA mixtures by designing two allele-specific primers. In this study, STR markers were selected from previously reports which had a high polymorphism in Chinese Han population. Then SNP markers with minor allele frequency (MAF) above 0.10 and locate no more than 250 bp from the repeat sequence of STR markers were screened from SNP database. Based on these criteria, eight SNP-STR markers were finally selected and amplification refractory mutation system (ARMS-PCR) method was used to achieve allele-specific amplification. 20 samples were used to estimate the polymorphism of SNPs in Chinese Han population. Six of the eight loci had a high polymorphism in SNP (above 0.20) which indicated the potential of discriminating DNA mixtures. Most of the primers target minor DNA at an excess of 100-fold major DNA. However, discrimination power is not high enough using these six loci. Therefore, further studies are necessary in developing more SNP-STR markers.
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影响因子：
3.9
作者：
Castella, Vincent;Gervaix, Joelle;Hall, Diana
通讯作者：
Hall, Diana
用于法医鉴定的 SNP-STR 多重分析的开发
DOI：
10.1016/j.fsigss.2015.09.236
发表时间：
2015-12-01
期刊：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES
影响因子：
--
作者：
Wang, L.;He, W.;Zhang, L.
通讯作者：
Zhang, L.
影响因子：
3.1
作者：
Lin, Sze-Wah;Li, Christina;Ip, Stephen C. Y.
通讯作者：
Ip, Stephen C. Y.
影响因子：
3.1
作者：
Oldoni, Fabio;Castella, Vincent;Hall, Diana
通讯作者：
Hall, Diana