Insights into long noncoding RNAs of naked mole rat (Heterocephalus glaber) and their potential association with cancer resistance.
Insights into long noncoding RNAs of naked mole rat (Heterocephalus glaber) and their potential association with cancer resistance.
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深入了解裸鼹鼠 (Heterocephalus glaber) 的长非编码 RNA 及其与抗癌性的潜在关联
DOI：
10.1186/s13072-016-0101-5
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发表时间：
2016
影响因子：
3.9
通讯作者：
Kong QP
中科院分区：
文献类型：
--
作者：
Jiang JJ;Cheng LH;Wu H;He YH;Kong QP
背景长非编码RNA(Long Non Coding RNAs，LncRNAs)是一类普遍存在的非编码RNA，具有肿瘤抑制基因或癌基因的作用，极大地改变了人们对癌症的认识。裸鼠是一种非常长寿和抗癌的啮齿动物，然而，在这个诱人的物种中，lncRNAs是否在抗癌中发挥作用尚不清楚。结果在本研究中，我们开发了一条管道，根据12个已发表的转录本，在核磁共振基因组中总共鉴定了4422个lncRNAs。系统分析表明，核磁共振lncRNAs与其他脊椎动物物种有许多共同的特征，如组织特异性和低表达。BLASTN与1057个人类癌症相关的lncRNAs的比对表明，只有5个核磁共振lncRNAs显示出同源性，这表明核磁共振lncRNAs与人类癌症相关的lncRNAs之间存在低序列保守性。进一步对lncRNAs和蛋白质编码基因的相关性分析表明，共有1295个lncRNAs与核磁共振中潜在的抑癌基因高度共表达(r≥为0.9orr≤为0.9cp值为0.90，cP值为0.01)，194个lncRNAs与4个高分子质量的透明质酸相关基因具有较强的相关性(r≤为0.8orr−为0.8orcP值为0.8cp值为≤为0.01)。结论本研究首次对核磁共振lncRNAs及其与肿瘤耐药性的关系进行了全面的全基因组分析。我们的结果提示，lncRNAs可能在核磁共振中的抗癌机制中有重要作用。
BackgroundLong noncoding RNAs (lncRNAs) are a class of ubiquitous noncoding RNAs and have been found to act as tumor suppressors or oncogenes, which dramatically altered our understanding of cancer. Naked mole rat (NMR,Heterocephalus glaber) is an exceptionally long-lived and cancer-resistant rodent; however, whether lncRNAs play roles in cancer resistance in this seductive species remains unknown.ResultsIn this study, we developed a pipeline and identified a total of 4422 lncRNAs across the NMR genome based on 12 published transcriptomes. Systematic analysis revealed that NMR lncRNAs share many common characteristics with other vertebrate species, such as tissue specificity and low expression. BLASTN against with 1057 human cancer-related lncRNAs showed that only 5 NMR lncRNAs displayed homology, demonstrating the low sequence conservation between NMR lncRNAs and human cancer-related lncRNAs. Further correlation analysis of lncRNAs and protein-coding genes indicated that a total of 1295 lncRNAs were intensively coexpressed (r≥ 0.9 orr≤ −0.9,cPvalue ≤ 0.01) with potential tumor-suppressor genes in NMR, and 194 lncRNAs exhibited strong correlation (r≥ 0.8 orr≤ −0.8,cPvalue ≤ 0.01) with four high-molecular-mass hyaluronan related genes that were previously identified to play key roles in cancer resistance of NMR.ConclusionIn this study, we provide the first comprehensive genome-wide analysis of NMR lncRNAs and their possible associations with cancer resistance. Our results suggest that lncRNAs may have important effects on anticancer mechanism in NMR.
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影响因子：
64.5
作者：
Huarte M;Guttman M;Feldser D;Garber M;Koziol MJ;Kenzelmann-Broz D;Khalil AM;Zuk O;Amit I;Rabani M;Attardi LD;Regev A;Lander ES;Jacks T;Rinn JL
通讯作者：
Rinn JL
影响因子：
3.9
作者：
Edrey, Yael H.;Park, Thomas J.;Buffenstein, Rochelle
通讯作者：
Buffenstein, Rochelle
影响因子：
16.6
作者：
Miyawaki S;Kawamura Y;Oiwa Y;Shimizu A;Hachiya T;Bono H;Koya I;Okada Y;Kimura T;Tsuchiya Y;Suzuki S;Onishi N;Kuzumaki N;Matsuzaki Y;Narita M;Ikeda E;Okanoya K;Seino K;Saya H;Okano H;Miura K
通讯作者：
Miura K
影响因子：
3.7
作者：
Kaushik K;Leonard VE;Kv S;Lalwani MK;Jalali S;Patowary A;Joshi A;Scaria V;Sivasubbu S
通讯作者：
Sivasubbu S
影响因子：
8.8
作者：
Hezroni H;Koppstein D;Schwartz MG;Avrutin A;Bartel DP;Ulitsky I
通讯作者：
Ulitsky I