Immobilization of horseradish peroxidase on self-assembled (3-mercaptopropyl)trimethoxysilane film: Characterization, direct electrochemistry, redox thermodynamics and biosensing
Immobilization of horseradish peroxidase on self-assembled (3-mercaptopropyl)trimethoxysilane film: Characterization, direct electrochemistry, redox thermodynamics and biosensing
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辣根过氧化物酶在自组装（3-巯基丙基）三甲氧基硅烷膜上的固定化：表征、直接电化学、氧化还原热力学和生物传感
DOI：
10.1016/j.electacta.2008.06.031
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发表时间：
2008-11
影响因子：
6.6
通讯作者：
Hu, Zhichao
中科院分区：
文献类型：
--
作者：
Wu, Fanghua;Jin, Litong;Xian, Yuezhong;Tian, Yuan;Wang, Liwei;Xu, Jingjing;Hu, Zhichao
采用溶胶-凝胶自组装技术制备了高度有序的3-巯丙基三甲氧基硅烷（3-MPT）薄膜。将辣根过氧化物酶（HRP）成功地固定在三维（3D）3-MPT网络上，实现了HRP的直接电化学反应。由还原电位的温度依赖性得到的HRP在3-MPT膜上的氧化还原热力学表明，HRP在3-MPT膜界面的氧化还原电位正移源于溶剂重组效应和HRP多肽链的构象变化。基于HRP的直接电化学和电催化能力，研制了一种灵敏的第三代电流型H2 O2生物传感器，其线性范围分别为5.0×10− 7 ~ 1.0×10− 4和1.0×10− 4 ~ 2.0×10− 2 molL −1。
Highly organized (3-mercaptopropyl)trimethoxysilane (3-MPT) films have been prepared via self-assembled coupled with sol–gel linking technology. Horseradish peroxidase (HRP) is successfully immobilized onto the densely packed three-dimensional (3D) 3-MPT network and the direct electrochemistry of HRP is achieved without any electron mediators or promoters. Redox thermodynamics of HRP on the 3-MPT films, which is obtained from the temperature dependence of the reduction potential, suggests that the positive shift of redox potentials of HRP at the interface of 3-MPT originates from the solvent reorganization effects and conformational change of the polypeptide chain of HRP. Based on the direct electrochemistry and electrocatalytic ability of HRP, a sensitive third-generation amperometric H2O2biosensor is developed with two linear dependence ranges of 5.0×10−7to 1.0×10−4and 1.0×10−4to 2.0×10−2molL−1.
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影响因子：
3.9
作者：
M. Cheung;D. Trau;K. Yeung;M. Carles;N. Sucher
通讯作者：
M. Cheung;D. Trau;K. Yeung;M. Carles;N. Sucher
DOI：
10.1002/chin.197922065
发表时间：
1979-02
期刊：
ChemInform
影响因子：
--
作者：
E. Yee;R. J. Cave;K. L. Guyer;P. Tyma;M. J. Weaver
通讯作者：
E. Yee;R. J. Cave;K. L. Guyer;P. Tyma;M. J. Weaver
影响因子：
5
作者：
Elizabeth V. Milsom;Hayley A Dash;A. Jenkins;M. Opallo;F. Marken
通讯作者：
Elizabeth V. Milsom;Hayley A Dash;A. Jenkins;M. Opallo;F. Marken
影响因子：
4.5
作者：
Jiang-Lan Li;Lihong Liu;Fei Xiao;Zhe Gui;Rui Yan;Faqiong Zhao;Ling Hu;Baizhao Zeng
通讯作者：
Jiang-Lan Li;Lihong Liu;Fei Xiao;Zhe Gui;Rui Yan;Faqiong Zhao;Ling Hu;Baizhao Zeng
DOI：
10.1002/chin.199834325
发表时间：
1998-05
期刊：
ChemInform
影响因子：
--
作者：
J. Rusling
通讯作者：
J. Rusling