Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis.
Bacterial community profiling of milk samples as a means to understand culture-negative bovine clinical mastitis.
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DOI：
10.1371/journal.pone.0061959
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Phillips GJ
中科院分区：
文献类型：
--
作者：
Kuehn JS;Gorden PJ;Munro D;Rong R;Dong Q;Plummer PJ;Wang C;Phillips GJ
牛乳腺的炎症和感染，通常称为乳腺炎，每年在世界范围内的乳制品工业中造成重大损失。虽然几种不同的细菌物种已被鉴定为乳腺炎的病原体，但许多临床乳腺炎病例即使在细菌生长富集后仍保持培养阴性。为了了解这种日益普遍的现象的基础，使用16 S核糖体RNA基因（16 S rDNA）扩增子的非培养物依赖性焦磷酸测序分析了来自牛奶样品的细菌群落的组成。比较了来自乳腺炎乳区的培养阴性乳样品与来自相同动物的非乳腺炎乳区的培养阴性乳样品的微生物群落组成。分离培养阴性临床和健康乳区样本对的基因组DNA，使用细菌16 S rRNA基因V1-V2区特异性索引引物制备扩增子文库，并使用Roche 454 GS FLX钛化学测序。对这些样品的分类组成的评估揭示了来自乳腺炎和健康乳区的乳汁中微生物群的显著差异。统计分析确定了7个细菌属，可能是主要负责观察到的微生物群落之间的差异乳腺炎和健康的季度。总的来说，这些结果提供了证据，即培养阴性乳腺炎病例可能与可能存在于低于本文使用的培养检测阈值的细菌种类相关。非培养细菌群落分析的应用是了解动物健康和疾病中长期存在的问题的一种强有力的方法。
Inflammation and infection of bovine mammary glands, commonly known as mastitis, imposes significant losses each year in the dairy industry worldwide. While several different bacterial species have been identified as causative agents of mastitis, many clinical mastitis cases remain culture negative, even after enrichment for bacterial growth. To understand the basis for this increasingly common phenomenon, the composition of bacterial communities from milk samples was analyzed using culture independent pyrosequencing of amplicons of 16S ribosomal RNA genes (16S rDNA). Comparisons were made of the microbial community composition of culture negative milk samples from mastitic quarters with that of non-mastitic quarters from the same animals. Genomic DNA from culture-negative clinical and healthy quarter sample pairs was isolated, and amplicon libraries were prepared using indexed primers specific to the V1–V2 region of bacterial 16S rRNA genes and sequenced using the Roche 454 GS FLX with titanium chemistry. Evaluation of the taxonomic composition of these samples revealed significant differences in the microbiota in milk from mastitic and healthy quarters. Statistical analysis identified seven bacterial genera that may be mainly responsible for the observed microbial community differences between mastitic and healthy quarters. Collectively, these results provide evidence that cases of culture negative mastitis can be associated with bacterial species that may be present below culture detection thresholds used here. The application of culture-independent bacterial community profiling represents a powerful approach to understand long-standing questions in animal health and disease.
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DOI：
10.1099/00207713-31-3-339
发表时间：
1981-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
JASPER, DE;ERNO, H;CHRISTIANSEN, C
通讯作者：
CHRISTIANSEN, C
影响因子：
2.5
作者：
Contreras, G. Andres;Miguel Rodriguez, Juan
通讯作者：
Miguel Rodriguez, Juan
影响因子：
4.4
作者：
Delbes, Celine;Ali-Mandjee, Leila;Montel, Marie-Christine
通讯作者：
Montel, Marie-Christine
影响因子：
9.8
作者：
Gevers D;Knight R;Petrosino JF;Huang K;McGuire AL;Birren BW;Nelson KE;White O;Methé BA;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
4.5
作者：
Huse SM;Dethlefsen L;Huber JA;Mark Welch D;Relman DA;Sogin ML
通讯作者：
Sogin ML