OFF bipolar cell density varies by subtype, eccentricity, and along the dorsal ventral axis in the mouse retina.
OFF bipolar cell density varies by subtype, eccentricity, and along the dorsal ventral axis in the mouse retina.
复制标题
OFF双极细胞密度因亚型、偏心率和沿着背腹轴而异。
DOI：
10.1002/cne.25064
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发表时间：
2021-06
期刊：
影响因子：
--
通讯作者：
Fuerst PG
中科院分区：
文献类型：
--
作者：
Camerino MJ;Engerbretson IJ;Fife PA;Reynolds NB;Berria MH;Doyle JR;Clemons MR;Gencarella MD;Borghuis BG;Fuerst PG
神经视网膜沿沿着中央-周边、背腹和层状平面组织。在灵长类、小鼠、鱼类和鸟类等物种中，细胞密度和分布沿着中央-外周轴和背侧-腹侧轴变化。视网膜内细胞类型的差异分布与对不同类型损伤的敏感性相关，这些损伤是主要视网膜疾病（包括黄斑变性和青光眼）的基础。在广泛使用的研究模型（包括小鼠）中，对于多种细胞类型，视网膜分布的正常变化仍未报道。在这里，我们绘制了所有已知的OFF双极细胞（BC）群体和水平细胞的分布。我们报告了沿着视网膜背腹轴和中心外周轴的OFF BC群体和水平细胞分布的显著变化。某些OFF BC细胞群的分布模式比其他细胞群明显得多，可能与细胞类型的专门功能相对应。OFF双极细胞的密度因亚型、偏心率和沿着背腹轴而异。(a)将视网膜分成四个象限，用短波视蛋白标记以确定方向。(b)将细胞标记并保存为一系列单独的坐标，这些坐标进行Voronoi镶嵌以分析空间异质性。(c)对体积数据集进行计数以确定细胞计数。(d)按亚型1a、1b、2、3a、3b、4列出的关闭双极细胞密度等值线图。
The neural retina is organized along central-peripheral, dorsal-ventral and laminar planes. Cellular density and distributions vary along the central-peripheral and dorsal-ventral axis in species including primates, mice, fish and birds. Differential distribution of cell types within the retina is associated with sensitivity to different types of damage that underpin major retinal diseases, including macular degeneration and glaucoma. Normal variation in retinal distribution remains unreported for multiple cell types in widely used research models, including mouse. Here we map the distribution of all known OFF bipolar cell (BC) populations and horizontal cells. We report significant variation in the distribution of OFF BC populations and horizontal cells along the dorsal-ventral and central-peripheral axes of the retina. Distribution patterns are much more pronounced for some populations of OFF BC cells than others and may correspond to the cell type’s specialized functions. Density of OFF bipolar cells vary by subtype, eccentricity and along the dorsal ventral axis. (a) Retinas were disected into four quadrants labeled with short-wave opsin to determine orientation. (b) Cells were marked and saved as series of individual coordinates which underwent Voronoi tessellation to analyze spatial heterogeneity. (c) Volumetric datasets were counted do determine cell count. (d) OFF-bipolar cell density contour maps by subtype 1a, 1b, 2, 3a, 3b, 4.
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影响因子：
--
作者：
APPLE D J
通讯作者：
APPLE D J
影响因子：
1.9
作者：
BOYCOTT, BB;HOPKINS, JM
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HOPKINS, JM
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作者：
Haverkamp, S;Wässle, H;Euler, T
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影响因子：
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作者：
Garrick, D;Fiering, S;Whitelaw, E
通讯作者：
Whitelaw, E