The actin-driven movement and formation of acetylcholine receptor clusters.
The actin-driven movement and formation of acetylcholine receptor clusters.
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DOI：
10.1083/jcb.150.6.1321
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发表时间：
2000-09-18
期刊：
影响因子：
--
通讯作者：
Peng HB
中科院分区：
文献类型：
--
作者：
Dai Z;Luo X;Xie H;Peng HB
设计了一种新的方法来显示由突触后分化信号诱导的培养肌肉细胞中肌动蛋白聚合。这需要掩蔽肌原纤维丝状（F）-肌动蛋白与jasplakinastatin，细胞渗透F-肌动蛋白结合毒素，突触刺激之前，然后探测新的肌动蛋白组装与荧光鬼笔环肽。与此程序，肌动蛋白聚合与新诱导的乙酰胆碱受体（AChR）的肝素结合的生长相关的分子包被珠和聚集蛋白的集群进行了观察。珠诱导局部F-肌动蛋白组装，共定位与AChR集群在珠-肌肉接触，而肌动蛋白细胞骨架和AChR集群诱导浴agrin应用是弥漫性的。通过表达一种绿色荧光蛋白偶联的corneum蛋白（一种与活性F-肌动蛋白结合的蛋白质），揭示了与新AChR簇相关的肌动蛋白细胞骨架的动态性质。事实上，肌动蛋白聚合产生的动力推动了整个珠子诱导的AChR簇及其附着的珠子在膜平面上移动。此外，肌动蛋白聚合对于珠粒和聚集蛋白诱导的AChR簇的形成以及磷酸酪氨酸积累也是必需的，如通过latrunculin A（一种螯合球状（G）-肌动蛋白并阻止F-肌动蛋白组装的毒素）对它们的阻断所示。这些结果表明，肌动蛋白聚合引起的突触信号是必要的AChR集群的运动和形成，并牵连的作用，F-肌动蛋白作为突触后支架的组装结构和信号分子在神经肌肉接头形成。
A new method was devised to visualize actin polymerization induced by postsynaptic differentiation signals in cultured muscle cells. This entails masking myofibrillar filamentous (F)-actin with jasplakinolide, a cell-permeant F-actin–binding toxin, before synaptogenic stimulation, and then probing new actin assembly with fluorescent phalloidin. With this procedure, actin polymerization associated with newly induced acetylcholine receptor (AChR) clustering by heparin-binding growth-associated molecule–coated beads and by agrin was observed. The beads induced local F-actin assembly that colocalized with AChR clusters at bead–muscle contacts, whereas both the actin cytoskeleton and AChR clusters induced by bath agrin application were diffuse. By expressing a green fluorescent protein–coupled version of cortactin, a protein that binds to active F-actin, the dynamic nature of the actin cytoskeleton associated with new AChR clusters was revealed. In fact, the motive force generated by actin polymerization propelled the entire bead-induced AChR cluster with its attached bead to move in the plane of the membrane. In addition, actin polymerization is also necessary for the formation of both bead and agrin-induced AChR clusters as well as phosphotyrosine accumulation, as shown by their blockage by latrunculin A, a toxin that sequesters globular (G)-actin and prevents F-actin assembly. These results show that actin polymerization induced by synaptogenic signals is necessary for the movement and formation of AChR clusters and implicate a role of F-actin as a postsynaptic scaffold for the assembly of structural and signaling molecules in neuromuscular junction formation.
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影响因子：
64.8
作者：
FORSCHER, P;LIN, CH;THOMPSON, C
通讯作者：
THOMPSON, C
DOI：
10.1083/jcb.110.6.2061
发表时间：
1990-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Chen Q;Sealock R;Peng HB
通讯作者：
Peng HB
影响因子：
3.5
作者：
Daggett, DF;Cohen, MW;Peng, HB
通讯作者：
Peng, HB
DOI：
10.1073/pnas.82.23.8270
发表时间：
1985-01-01
影响因子：
11.1
作者：
BURDEN, SJ
通讯作者：
BURDEN, SJ
影响因子：
4.8
作者：
Fuhrer, C;Hall, ZW
通讯作者：
Hall, ZW