Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme.
Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme.
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DOI：
10.1038/s41467-018-04437-9
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发表时间：
2018-06-06
影响因子：
16.6
通讯作者：
Yamazaki T
中科院分区：
文献类型：
--
作者：
Nishimura N;Tsuchiya W;Moresco JJ;Hayashi Y;Satoh K;Kaiwa N;Irisa T;Kinoshita T;Schroeder JI;Yates JR 3rd;Hirayama T;Yamazaki T
脱落酸（ABA）调节非生物胁迫和发育反应，包括调节种子休眠，以防止种子在不利的环境条件下发芽。ABA HYPERSENSITIVE GERMINATION1 （AHG1）编码2C型蛋白磷酸酶（PP2C），是ABA萌发反应的中心负调控因子；然而，AHG1的分子功能和调控尚不清楚。在这里，我们报道了AHG1与DELAY OF GERMINATION1 （DOG1）相互作用，后者是种子休眠的关键正调控因子。在体外实验中，DOG1作用于AHG1的上游，损害AHG1的PP2C活性。此外，DOG1具有结合血红素的能力。DOG1与AHG1和血红素的结合是独立的过程，但两者对于DOG1在体内的功能都是必不可少的。我们的研究表明，AHG1和DOG1通过整合多种环境信号，与PYL/RCAR ABA受体介导的调控系统并行，构成了一个重要的种子休眠和萌发调控系统。当条件不适宜时，激素脱落酸（ABA）会阻止种子发芽。在这里，作者表明，作为种子萌发的正调节因子，DOG1通过与AHG1磷酸酶的遗传和物理相互作用损害ABA信号，并且这种活性需要DOG1与血红素结合。
Abscisic acid (ABA) regulates abiotic stress and developmental responses including regulation of seed dormancy to prevent seeds from germinating under unfavorable environmental conditions. ABA HYPERSENSITIVE GERMINATION1 (AHG1) encoding a type 2C protein phosphatase (PP2C) is a central negative regulator of ABA response in germination; however, the molecular function and regulation of AHG1 remain elusive. Here we report that AHG1 interacts with DELAY OF GERMINATION1 (DOG1), which is a pivotal positive regulator in seed dormancy. DOG1 acts upstream of AHG1 and impairs the PP2C activity of AHG1 in vitro. Furthermore, DOG1 has the ability to bind heme. Binding of DOG1 to AHG1 and heme are independent processes, but both are essential for DOG1 function in vivo. Our study demonstrates that AHG1 and DOG1 constitute an important regulatory system for seed dormancy and germination by integrating multiple environmental signals, in parallel with the PYL/RCAR ABA receptor-mediated regulatory system. The hormone abscisic acid (ABA) prevents seeds from germination when conditions are not suitable. Here the authors show that DOG1, a positive regulator of germination, impairs ABA signaling via genetic and physical interactions with the AHG1 phosphatase and that DOG1 binding to heme is required for this activity.
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影响因子：
5.6
作者：
Arc E;Galland M;Godin B;Cueff G;Rajjou L
通讯作者：
Rajjou L
DOI：
10.1073/pnas.0912021106
发表时间：
2009-12-15
影响因子：
11.1
作者：
Geiger, Dietmar;Scherzer, Soenke;Hedrich, Rainer
通讯作者：
Hedrich, Rainer
影响因子：
7.2
作者：
Dekkers, Bas J. W.;He, Hanzi;Bentsink, Leonie
通讯作者：
Bentsink, Leonie
DOI：
10.1073/pnas.74.8.3203
发表时间：
1977-01-01
影响因子：
11.1
作者：
ARNOLD, WP;MITTAL, CK;MURAD, F
通讯作者：
MURAD, F
影响因子：
7.4
作者：
Dupeux, Florine;Antoni, Regina;Marquez, Jose A.
通讯作者：
Marquez, Jose A.