Temperature-Induced Inactivation of Cytoplasmic Biogel Osmosensing Properties is Associated with Suppression of Regulatory Volume Decrease in A549 Cells
Temperature-Induced Inactivation of Cytoplasmic Biogel Osmosensing Properties is Associated with Suppression of Regulatory Volume Decrease in A549 Cells
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温度诱导的细胞质生物凝胶渗透传感特性失活与 A549 细胞调节体积减少的抑制有关
DOI：
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发表时间：
2014
影响因子：
2.4
通讯作者：
S. Orlov
中科院分区：
文献类型：
--
作者：
A. Platonova;F. Boudreault;L. Kapilevich;G. Maksimov;O. Ponomarchuk;R. Grygorczyk;S. Orlov
参与细胞体积调节的细胞内信号传导的上游中间体仍然很少探索。最近，我们证明渗透压诱导的体积变化在透化细胞是几倍高于观察到的与完整的细胞，表明细胞质凝胶的渗透敏感特性。为了进一步研究细胞质热蛋白在细胞体积调节中的作用，我们比较了短期热处理对完整和透化的A549细胞体积变化的作用。在48 °C下预处理A549细胞抑制了由唐南平衡消散以及低渗培养基引发的肿胀。值得注意的是，热处理完全取消了hyposomotic介质对透化细胞体积变化的作用，表明温度升高通过其对细胞膜的影响而不是对质膜透性的影响来抑制细胞膜敏感特性。相同的热处理阻断了调节性容积减少（RVD）以及在暴露于低渗肿胀的对照细胞中观察到的Ba 2+敏感性K+通道活性的增加。与肿胀不同，在50 °C下预孵育10分钟的细胞中，高渗收缩减少了两倍。我们的研究结果表明，细胞质凝胶的细胞内感应是低渗肿胀触发RVD的关键事件。细胞膜和质膜在高渗收缩引发的细胞内信号传导中的作用有待进一步研究。
Upstream intermediates of intracellular signaling involved in cell volume regulation remain poorly explored. Recently, we demonstrated that osmolarity-induced volume changes in permeabilized cells were several-fold higher than those observed with intact cells, indicating the osmosensing properties of cytoplasmic gel. To further examine the role of cytoplasmic biogel in cell volume regulation, we compared the action of short-term heat treatment on volume changes in intact and permeabilized A549 cells. Pretreatment of A549 cells at 48 °C suppressed swelling triggered by dissipation of Donnan’s equilibrium as well as by hyposmotic medium. Significantly, heat treatment completely abolished the action of hyposomotic medium on volume changes in permeabilized cells, showing that temperature elevation suppresses osmosensing properties via its effect on biogel rather than on plasma membrane water permeability. Identical heat treatment blocked the regulatory volume decrease (RVD) as well as the increment of Ba2+-sensitive K+-channel activity seen in control cells exposed to hyposmotic swelling. Unlike swelling, hyperosmotic shrinkage was decreased by twofold in cells subjected to 10-min preincubation at 50 °C. Our results disclose that osmosensing by cytoplasmic gel is a key event in the RVD triggered by hypotonic swelling. The role of biogel and plasma membrane in intracellular signaling triggered by hyperosmotic shrinkage should be further investigated.
DOI：
10.1016/s0928-4680(01)00074-8
发表时间：
2001-12-01
期刊：
Pathophysiology : the official journal of the International Society for Pathophysiology
影响因子：
--
作者：
Mongin, Alexander A.;Orlov, Sergei N.
通讯作者：
Orlov, Sergei N.
P2Y 受体的激活导致 C11-MDCK 肾上皮细胞强烈且持续的收缩。
DOI：
10.1152/ajpcell.00018.2011
发表时间：
2011
期刊：
American journal of physiology. Cell physiology
影响因子：
--
作者：
Koltsova,SvetlanaV;Platonova,Alexandra;Maksimov,GeorgyV;Mongin,AlexanderA;Grygorczyk,Ryszard;Orlov,SergeiN
通讯作者：
Orlov,SergeiN