Controlled biodegradation of self-assembling β-hairpin peptide hydrogels by proteolysis with matrix metalloproteinase-13.
Controlled biodegradation of self-assembling β-hairpin peptide hydrogels by proteolysis with matrix metalloproteinase-13.
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DOI：
10.1016/j.biomaterials.2011.05.052
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发表时间：
2011-09
期刊：
影响因子：
14
通讯作者：
Schneider, Joel P.
中科院分区：
文献类型：
--
作者：
Giano, Michael C.;Pochan, Darrin J.;Schneider, Joel P.
将基质金属蛋白酶-13的生物降解控制纳入自组装β-发夹肽水凝胶的设计中。降解肽（DP肽）是一系列对MMP-13具有不同蛋白水解敏感性的五种肽。这些肽在生理相关条件下（150 mM NaCl, pH 7.6）进行环境触发折叠和自组装，形成自支撑水凝胶。在酶的存在下，由不同多肽制备的凝胶根据组成凝胶的单个肽的一级序列以不同的速率降解。在14天的过程中，通过振荡剪切流变学监测材料的降解情况，凝胶的总体降解率从5%到70%不等。降解产物采用高效液相色谱法分析，电喷雾电离质谱法鉴定。这些数据表明构成每个凝胶的亲本肽的蛋白水解发生在预定的序列位置。DP水凝胶对MMP-13具有特异性，并且只能被基质金属蛋白酶-3 (MMP-3)（组织损伤过程中存在的另一种常见酶）进行最小程度的切割。用SW1353细胞进行的体外迁移实验表明，通过每种凝胶的迁移率根据肽序列不同而不同，这与使用外源MMP-13进行蛋白水解的研究一致。
Controlled biodegradation specific to matrix metalloproteinase-13 was incorporated into the design of self-assembling β-hairpin peptide hydrogels. Degrading Peptides (DP peptides) are a series of five peptides that have varying proteolytic susceptibilities towards MMP-13. These peptides undergo environmentally triggered folding and self-assembly under physiologically relevant conditions (150 mM NaCl, pH 7.6) to form self supporting hydrogels. In the presence of enzyme, gels prepared from distinct peptides are degraded at rates that differ according to the primary sequence of the single peptide comprising the gel. Material degradation was monitored by oscillatory shear rheology over the course of 14 days, where overall degradation of the gels vary from 5% to 70%. Degradation products were analyzed by HPLC and identified by electrospray-ionization mass spectrometry. This data shows that proteolysis of the parent peptides constituting each gel occurs at the intended sequence location. DP hydrogels show specificity to MMP-13 and are only minimally cleaved by matrix metalloproteinase-3 (MMP-3), another common enzyme present during tissue injury. In vitro migration assays performed with SW1353 cells show that migration rates through each gel differs according to peptide sequence, which is consistent with the proteolysis studies using exogenous MMP-13.
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影响因子：
14
作者：
Branco, Monica C.;Pochan, Darrin J.;Wagner, Norman J.;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
DOI：
10.1016/b978-012370615-7/50080-9
发表时间：
2007-01-01
期刊：
PRINCIPLES OF TISSUE ENGINEERING, 3RD EDITION
影响因子：
--
作者：
Ghosh, Kaustabh;Clark, Richard A. F.
通讯作者：
Clark, Richard A. F.
DOI：
10.1073/pnas.0701980104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Haines-Butterick, Lisa;Rajagopal, Karthikan;Schneider, Joel P.
通讯作者：
Schneider, Joel P.
影响因子：
2.1
作者：
Khelfallah, Nawel S.;Decher, Gero;Mesini, Philippe J.
通讯作者：
Mesini, Philippe J.
影响因子：
14
作者：
Kretsinger, JK;Haines, LA;Schneider, JP
通讯作者：
Schneider, JP