Colonization and genetic diversification processes of Leishmania infantum in the Americas.
Colonization and genetic diversification processes of Leishmania infantum in the Americas.
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DOI：
10.1038/s42003-021-01658-5
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发表时间：
2021-01-29
影响因子：
5.9
通讯作者：
Cupolillo E
中科院分区：
文献类型：
--
作者：
Schwabl P;Boité MC;Bussotti G;Jacobs A;Andersson B;Moreira O;Freitas-Mesquita AL;Meyer-Fernandes JR;Telleria EL;Traub-Csekö Y;Vaselek S;Leštinová T;Volf P;Morgado FN;Porrozzi R;Llewellyn M;Späth GF;Cupolillo E
婴儿利什曼原虫引起内脏利什曼病，这是一种在殖民时期传入美洲的致命媒介传播疾病。此后，这种非本地锥虫寄生虫利用其他媒介建立了广泛的传播周期，人类感染已成为公共卫生的重大关切，特别是在巴西。最近在巴西L.婴儿基因组中发现了一个多千碱基缺失，这可能会降低对抗利什曼药物米特福辛的易感性。我们发现，携带缺失的菌株至少出现在巴西的15个州，并描述了多样性模式，表明这些菌株来自共同的祖先突变体，而不是来自反复发生的独立突变事件。我们还发现，通过与非缺失型菌株杂交，缺失位点和相关酶活性得以恢复。遗传交换在继发性接触地区很常见，但在密切相关的寄生虫之间也很常见。我们研究了这种复杂的乳杆菌婴儿种群结构背后的人口统计学和生态情景，并讨论了疾病控制的影响。Philipp Schwabl、Mariana boit<e:1>和他们的同事分析了126个利什曼原虫婴儿基因组，以确定寄生虫入侵历史的人口统计学和选择性后果是如何导致巴西复杂的种群遗传异质性的。他们的数据表明，在短期和远距离尺度上，种群扩张、二次接触和遗传交换事件之间存在着复杂的相互作用。这些过程似乎也是31号染色体上与耐药性相关的多基因缺失增殖的关键。
Leishmania infantum causes visceral leishmaniasis, a deadly vector-borne disease introduced to the Americas during the colonial era. This non-native trypanosomatid parasite has since established widespread transmission cycles using alternative vectors, and human infection has become a significant concern to public health, especially in Brazil. A multi-kilobase deletion was recently detected in Brazilian L. infantum genomes and is suggested to reduce susceptibility to the anti-leishmanial drug miltefosine. We show that deletion-carrying strains occur in at least 15 Brazilian states and describe diversity patterns suggesting that these derive from common ancestral mutants rather than from recurrent independent mutation events. We also show that the deleted locus and associated enzymatic activity is restored by hybridization with non-deletion type strains. Genetic exchange appears common in areas of secondary contact but also among closely related parasites. We examine demographic and ecological scenarios underlying this complex L. infantum population structure and discuss implications for disease control. Philipp Schwabl, Mariana Boité, and colleagues analyze 126 Leishmania infantum genomes to determine how demographic and selective consequences of the parasite’s invasive history have contributed to intricate population genetic heterogeneity across Brazil. Their data suggest a complex interplay of population expansion, secondary contact and genetic exchange events underlying diversity patterns at short and long-distance scales. These processes also appear pivotal to the proliferation of a drug resistance-associated multi-gene deletion on chromosome 31.
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影响因子：
6.4
作者：
Goodhead I;Capewell P;Bailey JW;Beament T;Chance M;Kay S;Forrester S;MacLeod A;Taylor M;Noyes H;Hall N
通讯作者：
Hall N
DOI：
10.4269/ajtmh.1999.61.380
发表时间：
1999-09-01
影响因子：
3.3
作者：
Belli, A;García, D;Harris, E
通讯作者：
Harris, E
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
DOI：
10.1126/science.1169464
发表时间：
2009-04-10
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Akopyants NS;Kimblin N;Secundino N;Patrick R;Peters N;Lawyer P;Dobson DE;Beverley SM;Sacks DL
通讯作者：
Sacks DL
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
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