Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector.
Demonstration of genetic exchange during cyclical development of Leishmania in the sand fly vector.
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DOI：
10.1126/science.1169464
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发表时间：
2009-04-10
期刊：
影响因子：
--
通讯作者：
Sacks DL
中科院分区：
文献类型：
--
作者：
Akopyants NS;Kimblin N;Secundino N;Patrick R;Peters N;Lawyer P;Dobson DE;Beverley SM;Sacks DL
遗传交换作为利什曼原虫广泛多样性的机制尚未得到证实。我们在这里报告的证据表明，利什曼原虫的无脊椎动物阶段能够与非洲锥虫描述的减数分裂过程相一致的性周期。杂交后代的产生携带来自两个亲本的完整基因组互补物，但kDNA大环来自一个亲本。交配只发生在白蛉媒介中，杂交种通过白蛉叮咬传播给哺乳动物宿主。遗传交换可能有助于自然种群的表型多样性，杂交后代的分析将有助于控制性状，如毒力，组织向性和耐药性的基因的定位克隆。
Genetic exchange as a mechanism underlying the extensive diversity of Leishmania parasites has not been shown. We report here evidence that the invertebrate stages of Leishmania are capable of a sexual cycle consistent with a meiotic process as described for African trypanosomes. Hybrid progeny were generated that bore full genomic complements from both parents, but kDNA maxicircles from one parent. Mating occurred only in the sand fly vector, and hybrids were transmitted to the mammalian host by sand fly bite. Genetic exchange likely contributes to phenotypic diversity in natural populations, and analysis of hybrid progeny will be useful for positional cloning of the genes controlling traits such as virulence, tissue tropism, and drug resistance.
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