Early specification of intestinal epithelium in the chicken embryo: A study on the localization and regulation of CdxA expression
Early specification of intestinal epithelium in the chicken embryo: A study on the localization and regulation of CdxA expression
复制标题
鸡胚肠上皮的早期规范：CdxA 表达定位和调控的研究
DOI：
10.1046/j.1440-169x.1997.t01-4-00012.x
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发表时间：
1997
期刊：
影响因子：
4.6
通讯作者：
S. Yasugi
中科院分区：
文献类型：
--
作者：
Y. Ishii;K. Fukuda;H. Saiga;S. Matsushita;S. Yasugi
CdxA 是一种与果蝇尾部相关的鸡同源盒基因，与内胚层的区域化有关。据报道，在鸡胚胎的发育过程中，CdxA 表达在内胚层中出现，在发育第 1.5 天时在内胚层两侧形成双侧条带，随后形成肠上皮。随着肠管形成的进展，CdxA 表达区域向内侧和尾部延伸。还表明，在发育第 3 天后，CdxA 表达的吻端界限划定了胃和十二指肠之间的边界。 CdxA 在通向肠道的消化道附件中不表达，例如胰腺、肝脏和尿囊。肠道谱系中 CdxA 表达的早期限制表明，涉及 CdxA 表达的肠道规范在肠管形成之前就开始了。 CdxA 在上皮间质组织重组体中的表达表明间充质影响调节 CdxA 表达在确定胃和肠之间的边界方面发挥着重要作用。 CdxA 转录物空间分布的时间变化和组织重组实验的结果，以及早期内胚层和内脏中胚层的精确命运图谱，共同构建了肠道特化的机制模型。
CdxA, a chicken homeobox‐containing gene related to caudal in Drosophila, has been implicated in the regionalization of endoderm. It is reported here that, in the development of the chicken embryo, CdxA expression appears in the endoderm at day 1.5 of development as bilateral bands on either side of the splanchnopleure which later contribute to intestinal epithelium. The CdxA‐expressing area extends medially and caudally as formation of the gut tube progresses. It is also shown that the rostral limit of CdxA expression demarcates the boundary between stomach and duodenum after day 3 of development. CdxA is not expressed in digestive tract appendages which open into the intestine, such as pancreas, liver and allantois. Early restriction of CdxA expression in intestinal lineage suggests that the intestinal specification involving CdxA expression commences before the gut tube is formed. The expression of CdxA in epithelial‐mesenchymal tissue recombinants suggests that mesenchymal influence regulating CdxA expression plays an important role in confirming the boundary between the stomach and intestine. Chronological change in the spatial distribution of CdxA transcripts and the results of tissue recombination experiments, together with precise fate maps of early endoderm and splanchnic mesoderm, lead to a model of mechanisms by which intestinal specification is brought about.
影响因子：
56.9
作者：
BLUMBERG, B;WRIGHT, CVE;CHO, KWY
通讯作者：
CHO, KWY