Notch Signaling Regulates Muscle Stem Cell Homeostasis and Regeneration in a Teleost Fish.
Notch Signaling Regulates Muscle Stem Cell Homeostasis and Regeneration in a Teleost Fish.
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DOI：
10.3389/fcell.2021.726281
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发表时间：
2021
影响因子：
5.5
通讯作者：
Knight RD
中科院分区：
文献类型：
--
作者：
Sultan SHA;Dyer C;Knight RD
肌肉再生是由表达Pax7的常驻肌肉卫星细胞（muSC）的活性介导的。在小鼠中，Notch信号传导在静止期间调节muSC并在再生中促进muSC增殖。目前还不清楚Notch在调节muSC生物学中的这些作用是否在脊椎动物中是保守的，或者是哺乳动物的特异性特征。因此，我们研究了Notch在调节硬骨鱼（斑马鱼）muSC稳态和再生中的作用。我们还测试了muSC在肌节发育期间是否对Notch显示出不同的敏感性。在没有损伤的情况下，Notch对于防止垂直肌间隔处的muSC增殖是重要的。相比之下，Notch信号传导促进增殖并阻止损伤背景下的分化。Notch是早期和晚期幼虫阶段对损伤的增殖反应所必需的，这表明它在发育和成体阶段调节muSCs中起着类似的作用。我们的研究结果揭示了Notch信号在调节体内稳态下的muSC和促进硬骨鱼再生过程中的增殖中的保守作用。
Muscle regeneration is mediated by the activity of resident muscle satellite cells (muSCs) that express Pax7. In mouse Notch signaling regulates muSCs during quiescence and promotes muSC proliferation in regeneration. It is unclear if these roles of Notch in regulating muSC biology are conserved across vertebrates or are a mammalian specific feature. We have therefore investigated the role of Notch in regulating muSC homeostasis and regeneration in a teleost fish, the zebrafish. We have also tested whether muSCs show differential sensitivity to Notch during myotome development. In an absence of injury Notch is important for preventing muSC proliferation at the vertical myoseptum. In contrast, Notch signaling promotes proliferation and prevents differentiation in the context of injury. Notch is required for the proliferative response to injury at early and later larval stages, suggesting it plays a similar role in regulating muSCs at developing and adult stages. Our results reveal a conserved role for Notch signaling in regulating muSCs under homeostasis and for promoting proliferation during regeneration in teleost fish.
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影响因子：
7.7
作者：
Ganassi M;Badodi S;Wanders K;Zammit PS;Hughes SM
通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
21.3
作者：
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通讯作者：
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影响因子：
2.7
作者：
Berberoglu MA;Gallagher TL;Morrow ZT;Talbot JC;Hromowyk KJ;Tenente IM;Langenau DM;Amacher SL
通讯作者：
Amacher SL
DOI：
10.1007/978-1-60761-938-3_18
发表时间：
2011
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Eliceiri, Brian P;Gonzalez, Ana Maria;Baird, Andrew
通讯作者：
Baird, Andrew