Expression Pattern of Enkephalinergic Neurons in the Developing Spinal Cord Revealed by Preproenkephalin-Green Fluorescent Protein Transgenic Mouse and Its Colocalization with GABA Immunoreactivity
Expression Pattern of Enkephalinergic Neurons in the Developing Spinal Cord Revealed by Preproenkephalin-Green Fluorescent Protein Transgenic Mouse and Its Colocalization with GABA Immunoreactivity
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前脑啡肽原绿色荧光蛋白转基因小鼠揭示发育中脊髓中脑啡肽能神经元的表达模式及其与 GABA 免疫反应性的共定位
DOI：
10.1159/000321403
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发表时间：
2010-12
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
为了更好地了解脑啡肽（ENK）在脊髓中的个体发育功能，有必要清楚ENK能神经元的发育模式。为了解决这个问题，我们使用转基因小鼠，通过在前脑啡肽原（PPE）基因的特异性转录控制下表达增强的绿色荧光蛋白（GFP），很容易显示ENKergic神经元。GFP阳性神经元最早出现在胚胎第11.5天（E）的腹侧灰质的腹内侧部分。在E13，它们主要存在于中间区。此后，GFP阳性神经元的数量逐渐增加，并从腹侧延伸到背侧区域。定量分析表明，GFP阳性神经元的数量在出生后第7天达到高峰（P）。GFP阳性神经元数量在P3时达到高峰。P21时，GFP免疫反应阳性细胞的分布模式与成人脊髓相似。双标记结果显示，约三分之一的总γ-氨基丁酸细胞群与GFP共定位：E16时为34.9 ± 3.5%，P3时为32.4 ± 3.7%。双标记神经元占GFP阳性神经元的近一半：E16时为42.4 ± 2.4%，P3时为44.1 ± 2.9%。两者合计，目前的结果表明，ENKergic神经元的发展根据rostrocaudal和ventrodorthoral梯度。这些结果对于理解ENKergic神经传递在发育中的脊髓中的功能作用具有广泛的意义。
To gain better insight into the ontogenic function of enkephalin (ENK) in the spinal cord, it is necessary to have a clear picture of the developing pattern of the ENKergic neurons. To address this question, we used transgenic mice which reveal ENKergic neurons easily by expressing enhanced green fluorescent protein (GFP) under the specific transcriptional control of the preproenkephalin (PPE) gene. GFP-positive neurons first appeared at embryonic day (E) 11.5 in the ventromedial part of the cervical ventral gray matter. At E13, they were mainly present in the intermediate zone. Thereafter, GFP-positive neurons increased progressively in number and extended from ventral to dorsal regions. Quantitative analysis showed that GFP-positive neurons peaked in number at postnatal day (P) 7 at the cervical level. The number of GFP-positive neurons reached a peak at P3 at the lumbar level. At P21, the distribution pattern of GFP immunoreactivity was similar to that in the adult spinal cord. Double-labeling results showed that about one-third of the total γ-aminobutyric acid cell population colocalized with GFP: 34.9 ± 3.5% at E16 and 32.4 ± 3.7% at P3. Double-labeled neurons accounted for nearly half of the GFP-positive neurons: 42.4 ± 2.4% at E16 and 44.1 ± 2.9% at P3. Taken together, the present results suggest that ENKergic neurons develop according to a rostrocaudal and ventrodorsal gradient. These results have broad implications for understanding the functional roles of ENKergic neurotransmission in the developing spinal cord.
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影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
DOI：
--
发表时间：
1992
期刊：
--
影响因子：
--
作者：
Michael W. Miller
通讯作者：
Michael W. Miller
影响因子：
3.4
作者：
Nakamura, Kouichi;Watakabe, Akiya;Kaneko, Takeshi
通讯作者：
Kaneko, Takeshi
影响因子：
3.4
作者：
KouichiC . Nakamura;A. Watakabe;H. Hioki;F. Fujiyama;Yasuyo H. Tanaka;T. Yamamori;T. Kaneko
通讯作者：
KouichiC . Nakamura;A. Watakabe;H. Hioki;F. Fujiyama;Yasuyo H. Tanaka;T. Yamamori;T. Kaneko
影响因子：
3.4
作者：
Koshimizu, Yoshinori;Wu, Sheng-Xi;Kaneko, Takeshi
通讯作者：
Kaneko, Takeshi