Perinatal hyperoxia exposure impairs hypoxia-induced depolarization in rat carotid body glomus cells.
Perinatal hyperoxia exposure impairs hypoxia-induced depolarization in rat carotid body glomus cells.
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DOI：
10.1016/j.resp.2013.04.016
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发表时间：
2013-08-01
影响因子：
2.3
通讯作者：
Donnelly, David F.
中科院分区：
文献类型：
--
作者：
Kim, Insook;Yang, Dongjin;Carroll, John L.;Donnelly, David F.
慢性产后高氧通过降低颈动脉体对急性缺氧的敏感性来减少缺氧通气反应，如传入神经反应减少、颈动脉体球细胞钙反应减少以及对急性缺氧反应的儿茶酚胺分泌减少所证明。本研究检查高氧是否会改变血管球细胞的电生理特征。从 P1 或 P7 开始对大鼠进行高氧处理 1 周，从 P1 开始处理 2 周，然后收获并解离其颈动脉体以进行全细胞、穿孔贴片记录。与来自常氧动物的血管球细胞相比，高氧处理的细胞显示出响应缺氧和缺氧的去极化程度显着降低，尽管对20 mM K+的去极化反应几乎没有变化。与其他处理组和常氧处理的细胞相比，从 P1 到 P15 暴露于高氧的大鼠的血管球细胞的静息细胞膜电位在 P15 进行研究时略有去极化，但根据细胞大小标准化的电导在各组之间没有差异。我们得出的结论是，出生后高氧会损害颈动脉化学感受器缺氧转导，处于缺氧感应和膜去极化之间的步骤。这种情况的发生没有使基线电生理学特征发生重大变化，表明信号传导改变或不同渗漏通道亚型的相对丰度发生改变。
Chronic post-natal hyperoxia reduces the hypoxic ventilatory response by reducing the carotid body sensitivity to acute hypoxia as demonstrated by a reduced afferent nerve response, reduced calcium response of carotid body glomus cells and reduced catecholamine secretion in response to acute hypoxia. The present study examined whether hyperoxia alters the electrophysiological characteristics of glomus cells. Rats were treated with hyperoxia for 1 week starting at P1 or P7 and for 2 weeks starting at P1 followed by harvesting and dissociation of their carotid bodies for whole cell, perforated-patch recording. As compared to glomus cells from normoxia animals, hyperoxia treated cells showed a significant reduction in the magnitude of depolarization in response to hypoxia and anoxia, despite little change in the depolarizing response to 20 mM K+. Resting cell membrane potential in glomus cells from rats exposed to hyperoxia from P1 to P15 and studied at P15 was slightly depolarized compared to other treatment groups and normoxia-treated cells, but conductance normalized to cell size was not different among groups. We conclude that postnatal hyperoxia impairs carotid chemoreceptor hypoxia transduction at a step between hypoxia sensing and membrane depolarization. This occurs without a major change in baseline electrophysiological characteristics, suggesting altered signaling or alterations in the relative abundance of different leak channel isoforms.
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影响因子：
2.3
作者：
Bavis, Ryan W.;Kim, Insook;Pradhan, Nelish;Nawreen, Nawshaba;Dmitrieff, Elizabeth F.;Carroll, John L.;Donnelly, David F.
通讯作者：
Donnelly, David F.
影响因子：
3.3
作者：
Donnelly, DF;Kim, I;Carroll, JL
通讯作者：
Carroll, JL
影响因子：
5.5
作者：
Kim, Donghee;Cavanaugh, Eric J.;Carroll, John L.
通讯作者：
Carroll, John L.
影响因子：
3.3
作者：
Bisgard, GW;Olson, EB;Mitchell, GS
通讯作者：
Mitchell, GS
影响因子：
2.3
作者：
Wasicko, M. J.;Breitwieser, G. E.;Carroll, J. L.
通讯作者：
Carroll, J. L.