Regulation of oxygen transport during brain activation: stimulus-induced hemodynamic responses in human and animal cortices
Regulation of oxygen transport during brain activation: stimulus-induced hemodynamic responses in human and animal cortices
复制标题
大脑激活过程中氧运输的调节：刺激引起的人类和动物皮质的血流动力学反应
DOI：
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
T. Yanagida
中科院分区：
文献类型：
--
作者：
A. Seiyama;J. Seki;H. Tanabe;Y. Ooi;Y. Satomura;H. Fujisaki;T. Yanagida
背景神经元活动的区域变化与血流动力学变化之间的相关性是功能性磁共振成像（fMRI）和近红外光学成像（NIOI）等无创神经成像技术的一个主要问题。人们认为这些变化的紧密结合可以从这些技术获得的数据中阐明大脑功能。在本研究中，我们研究了视觉刺激期间人类枕叶皮层和周围神经刺激期间大鼠体感皮层神经元活动与血流动力学反应之间的关系。方法通过使用功能磁共振成像和 NIOI 进行同步测量，研究了人类枕叶皮层宏观血流动力学反应对视觉刺激的时间频率依赖性。通过分析膜电位（荧光）、血流动力学参数（可见光谱和激光多普勒血流测定法）和血管直径（图像分析仪），在动物模型中研究了微观血流动力学变化和神经元活动因周围神经刺激而变化的刺激强度依赖性。结果在一定水平的刺激强度上，局部脑血流量（rCBF）的增加伴随着局部脑血容量（rCBV）的减少，即， rCBF 和 rCBV 反应的分离在人类和动物实验中均发生。此外，动物实验表明，增加的rCBF和O2的分布远远超出了神经元激活区域，并且激活区域的增加显示出饱和。结论这些结果表明，在一定水平的神经元活动之上，区域脑血流（rCBF）和rCBV之间的调节机制起作用，以防止过量的O2流入局灶性激活区域。
BackgroundThe correlation between regional changes in neuronal activity and changes in hemodynamics is a major issue for noninvasive neuroimaging techniques such as functional magnetic resonance imaging (fMRI) and near-infrared optical imaging (NIOI). A tight coupling of these changes has been assumed to elucidate brain function from data obtained with those techniques. In the present study, we investigated the relationship between neuronal activity and hemodynamic responses in the occipital cortex of humans during visual stimulation and in the somatosensory cortex of rats during peripheral nerve stimulation.MethodsThe temporal frequency dependence of macroscopic hemodynamic responses on visual stimuli was investigated in the occipital cortex of humans by simultaneous measurements made using fMRI and NIOI. The stimulus-intensity dependence of both microscopic hemodynamic changes and changes in neuronal activity in response to peripheral nerve stimulation was investigated in animal models by analyzing membrane potential (fluorescence), hemodynamic parameters (visible spectra and laser-Doppler flowmetry), and vessel diameter (image analyzer).ResultsAbove a certain level of stimulus-intensity, increases in regional cerebral blood flow (rCBF) were accompanied by a decrease in regional cerebral blood volume (rCBV), i.e., dissociation of rCBF and rCBV responses occurred in both the human and animal experiments. Furthermore, the animal experiments revealed that the distribution of increased rCBF and O2 spread well beyond the area of neuronal activation, and that the increases showed saturation in the activated area.ConclusionsThese results suggest that above a certain level of neuronal activity, a regulatory mechanism between regional cerebral blood flow (rCBF) and rCBV acts to prevent excess O2 inflow into the focally activated area.
影响因子：
56.9
作者：
FOX, PT;RAICHLE, ME;DENCE, C
通讯作者：
DENCE, C
DOI：
10.1073/pnas.89.12.5675
发表时间：
1992-06-15
影响因子：
11.1
作者：
KWONG, KK;BELLIVEAU, JW;ROSEN, BR
通讯作者：
ROSEN, BR
影响因子：
2.5
作者：
FOX, PT;RAICHLE, ME
通讯作者：
RAICHLE, ME