Immunoblotting and Dot Immunobinding
Immunoblotting and Dot Immunobinding
复制标题
免疫印迹和点免疫结合
DOI：
10.1007/978-3-642-69943-6_14
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Y. Sun
中科院分区：
文献类型：
--
作者：
J. Gordon;C. Rordorf;M. Rosenthal;Y. Sun
近年来免疫分析技术的巨大进步得益于固相方法的出现。使用与固相结合的反应物可以通过简单、快速的洗涤程序轻松去除过量的试剂。由此产生的方法快速、简单且定量。然而，这种方法本身并不能直接给出有关免疫反应物种的同质性、纯度或身份的定性信息。经典的免疫扩散和免疫电泳依赖于凝胶相中的扩散和沉淀，确实产生了这样的定性信息。然而，这些方法缓慢、繁琐且不灵敏。随着免疫印迹的出现（Towbin 等人，1979），将定性分析的经典方法的强大功能与固相方法的速度、简单性和灵敏度结合起来已经成为可能。
The great advances in immunoassay technique in recent years have resulted from the advent of solid-phase methodology. The use of reactants bound to a solid phase permits the easy removal of excess reagents in simple, rapid washing procedures. The resulting methodology is rapid, simple, and quantitative. However, this methodology does not as such give directly qualitative information concerning homogeneity, purity, or identity of immunoreacting species. Classic immuno-diffusion and immunoelectrophoresis, depending on diffusion and precipitation in a gel phase, did indeed yield such qualitative information. However, these methods are slow, cumbersome, and insensitive. With the advent of immunoblotting (Towbin et al. 1979), it has been possible to combine the power of the classic methodology for qualitative analysis with the speed, simplicity, and sensitivity of solid-phase methodology.
人-人杂交瘤产生的单克隆狼疮自身抗体的多特异性。
DOI：
10.1056/nejm198302243080802
发表时间：
1983
期刊：
The New England journal of medicine
影响因子：
--
作者：
Shoenfeld,Y;Rauch,J;Massicotte,H;Datta,SK;Andre-Schwartz,J;Stollar,BD;Schwartz,RS
通讯作者：
Schwartz,RS