Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas.
Polyspecificity of monoclonal lupus autoantibodies produced by human-human hybridomas.
复制标题
人-人杂交瘤产生的单克隆狼疮自身抗体的多特异性。
DOI：
10.1056/nejm198302243080802
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Schwartz,RS
中科院分区：
文献类型：
--
作者：
Shoenfeld,Y;Rauch,J;Massicotte,H;Datta,SK;Andre-Schwartz,J;Stollar,BD;Schwartz,RS
我们研究了系统性红斑狼疮患者淋巴细胞杂交瘤产生的单克隆自身抗体的血清学特性。杂交瘤是通过将人淋巴母细胞系 GM 4672（源自多发性骨髓瘤患者）与六名狼疮患者的外周血或脾淋巴细胞融合而制成的。分析了 30 种单克隆自身抗体，这些抗体因其与变性 DNA 反应的能力而被选择。其中 18 个与三个或更多其他多核苷酸发生反应，包括天然 DNA、左手双螺旋 DNA (Z-DNA)、聚 (I) 和聚 (dT)。十个与核酸和磷脂心磷脂均发生反应。单克隆自身抗体的多重结合反应可以通过多核苷酸和磷脂中存在适当间隔的磷酸二酯基团来解释。不同性质的聚合物共享抗原基团可能导致系统性红斑狼疮血清学反应的明显多样性。这些发现表明，DNA 本身不一定是这种疾病中自身抗体形成的免疫原性刺激物。 （新英格兰医学杂志，1983 年；308：414–20。）
We studied the serologic properties of monoclonal autoantibodies that were produced by hybridomas derived from lymphocytes of patients with systemic lupus erythematosus. The hybridomas were made by fusion of a human lymphoblastoid cell line, GM 4672 (derived from a patient with multiple myeloma), with peripheral-blood or splenic lymphocytes from six patients with lupus. Thirty monoclonal autoantibodies, selected for their ability to react with denatured DNA, were analyzed. Eighteen of them reacted with three or more additional polynucleotides, including native DNA, left-handed double-helical DNA (Z-DNA), poly(I), and poly(dT). Ten reacted both with nucleic acids and the phospholipid cardiolipin. The multiple binding reactions of the monoclonal autoantibodies may be explained by the presence of appropriately spaced phosphodiester groups in both the polynucleotides and the phospholipid. The sharing of antigenic groups by polymers of different natures may contribute to the apparent diversity of serologic reactions in systemic lupus erythematosus. These findings suggest that DNA itself need not be the immunogenic stimulus for autoantibody formation in this disease. (N Engl J Med. 1983; 308:414–20.)
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