A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.
A trehalose biosynthetic enzyme doubles as an osmotic stress sensor to regulate bacterial morphogenesis.
复制标题
海藻糖生物合成酶兼作渗透应激传感器来调节细菌形态发生
DOI：
10.1371/journal.pgen.1007062
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发表时间：
2017-10
期刊：
影响因子：
4.5
通讯作者：
Dyson P
中科院分区：
文献类型：
--
作者：
Chen X;An L;Fan X;Ju F;Zhang B;Sun H;Xiao J;Hu W;Qu T;Guan L;Tang S;Chen T;Liu G;Dyson P
二糖海藻糖是一种重要的细胞内渗透保护剂，OtsA/B 途径是多种细菌物种海藻糖生物合成的主要途径。支架蛋白和其他细胞骨架元件在细菌的形态发生过程中发挥着重要作用。在这里，我们描述了 OtsA 除了在海藻糖生物合成中的作用之外，还如何充当渗透压传感器来调节节杆菌 A3 菌株的细胞形态。为了应对渗透压，该节杆菌和其他节杆菌经历从杆菌生长到菌丝体生长的转变。 otsA 无效突变体表现出组成型菌丝体生长。渗透胁迫导致海藻糖-6-磷酸（OtsA 酶的产物）的消耗，并且这种代谢物的实验消耗也会导致不依赖于 OtsA 功能的组成型菌丝体生长。体外分析表明，在 6-磷酸海藻糖的促进下，OtsA 可以自组装成蛋白质网络，而大肠杆菌中的等效酶则不具备这一特性，尽管后者在节杆菌中表达时具有酶活性。这以及该蛋白在非应激细胞中细胞中部和两极的定位表明，来自节杆菌的 OtsA 可能起到调节细胞形态的细胞骨架元件的作用。为这种形态发生功能招募生物合成酶代表了可以在极端环境中生存的细菌的一种有趣的适应。
The dissacharide trehalose is an important intracellular osmoprotectant and the OtsA/B pathway is the principal pathway for trehalose biosynthesis in a wide range of bacterial species. Scaffolding proteins and other cytoskeletal elements play an essential role in morphogenetic processes in bacteria. Here we describe how OtsA, in addition to its role in trehalose biosynthesis, functions as an osmotic stress sensor to regulate cell morphology in Arthrobacter strain A3. In response to osmotic stress, this and other Arthrobacter species undergo a transition from bacillary to myceloid growth. An otsA null mutant exhibits constitutive myceloid growth. Osmotic stress leads to a depletion of trehalose-6-phosphate, the product of the OtsA enzyme, and experimental depletion of this metabolite also leads to constitutive myceloid growth independent of OtsA function. In vitro analyses indicate that OtsA can self-assemble into protein networks, promoted by trehalose-6-phosphate, a property that is not shared by the equivalent enzyme from E. coli, despite the latter’s enzymatic activity when expressed in Arthrobacter. This, and the localization of the protein in non-stressed cells at the mid-cell and poles, indicates that OtsA from Arthrobacter likely functions as a cytoskeletal element regulating cell morphology. Recruiting a biosynthetic enzyme for this morphogenetic function represents an intriguing adaptation in bacteria that can survive in extreme environments.
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影响因子：
4.2
作者：
Alvarez, HM;Silva, RA;Steinbüchel, A
通讯作者：
Steinbüchel, A
影响因子：
4.8
作者：
Gibson, RP;Tarling, CA;Davies, GJ
通讯作者：
Davies, GJ
影响因子：
3.5
作者：
KAASEN, I;MCDOUGALL, J;STROM, AR
通讯作者：
STROM, AR
影响因子：
21.3
作者：
Ingerson-Mahar, Michael;Briegel, Ariane;Werner, John N.;Jensen, Grant J.;Gitai, Zemer
通讯作者：
Gitai, Zemer
影响因子：
3.2
作者：
GERMIDA, JJ;CASIDA, LE
通讯作者：
CASIDA, LE