123I-MIBG pulmonary removal: a biochemical marker of minimal lung endothelial cell lesions
123I-MIBG pulmonary removal: a biochemical marker of minimal lung endothelial cell lesions
复制标题
123I-MIBG 肺切除：最小肺内皮细胞病变的生化标志物
DOI：
--
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
A. Donath
中科院分区：
文献类型：
--
作者：
D. Slosman;B. Polla,;A. Donath
循环生物胺，如血清素或去甲肾上腺素，由肺清除，可以被认为是肺内皮完整性的指标。碘化去甲肾上腺素类似物，间碘苄胍（MIBG），是通过一个积极的，饱和的运输系统在肺中提取。为了研究使用MIBG肺提取物检测最小的肺内皮细胞损伤，在大鼠中使用每天重复腹膜内注射博来霉素（BLM：2U/100 g体重）在5天的时间内开发了初始肺血管损伤的实验模型。此时，观察到血清血管紧张素转换酶活性（内皮细胞代谢的指标）显著降低（对照组为214±11 U/ml，BLM处理组为182±11 U/ml，P < 0.05）;电子显微镜显示存在典型但极轻微的内皮细胞损伤（起泡）。在离体肺灌流模型上，123 I-MIBG（0.1 μCi/ml）和125 I标记的人血清白蛋白（HSA）（0.2 μCi/ml）分别灌流10 min和2 min后，测定MIBG提取率（%EMIBG）。大鼠腹腔注射BLM 5天后，MIBG的肺提取量显著减少。%EMIBG从对照组的对照值24.7%±1.1%下降至BLM处理组的19.3%±1.6%（n=27，P<0.05; -21.2%）。HSA肺提取物，用作非特异性残留肺活性的估计，是没有不同的BLM处理大鼠的肺与对照组相比。由于MIBG肺提取物（%EMIBG）的测量能够证明由于BLM毒性导致的最小内皮细胞损伤的存在，因此MIBG是肺内皮细胞功能的指标。
Circulating biogenic amines, such as serotonin or norepinephrine, are cleared by the lung and can be considered as indicators of pulmonary endothelial integrity. An iodinated norepinephrine analogue, metaiodo-benzylguanidine (MIBG), is extracted in the lung by an active, saturable transport system. In order to investigate the use of MIBG lung extraction to detect minimal,lung endothelial cell lesions, an experimental model of initial pulmonary vascular lesions was developed in rats using repeated daily intraperitoneal injection of bleomycin (BLM: 2 U/100 g body weight) over a 5-day period. At this time, a significant decrease of serum angiotensin-converting enzyme activity, an index of endothelial cell metabolism, was observed (214±11 U/ml in controls as compared with 182±11 U/ml in BLM-treated rats,P < 0.05); electron microscopy showed the presence of typical but minimal endothelial cell lesions (blebbing). MIBG extraction (%EMIBG) was measured using the isolated perfused lung model after a 10-min steady-state period and 2 min of perfusion with123I-MIBG (0.1 μCi/ml) and125I-labelled human serum albumin (HSA) (0.2 μCi/ml). Intraperitoneal administration of BLM to rats for 5 days resulted in a significant decrease in pulmonary extraction of MIBG. %EMIBG declined from a control value of 24.7%±1.1% in controls to 19.3%±1.6% in BLM-treated rats (n=27,P<0.05; -21.2%). HSA lung extraction, used as an estimate of nonspecific residual lung activity, was not different in the lungs of BLM-treated rats as compared with controls. Because measurements of MIBG lung extraction (%EMIBG) were able to demonstrate the presence of minimal endothelial cell lesions due to the BLM toxicity, MIBG is an index of pulmonary endothelial cell function.
囊性纤维化和长期机械通气后蛙皮素、降钙素和血清素免疫反应性肺神经内分泌细胞的变化。
DOI：
10.1164/ajrccm/137.1.123
发表时间：
1988
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Johnson,DE;Wobken,JD;Landrum,BG
通讯作者：
Landrum,BG