Glyoxalase-I is a novel prognosis factor associated with gastric cancer progression.
Glyoxalase-I is a novel prognosis factor associated with gastric cancer progression.
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DOI：
10.1371/journal.pone.0034352
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Lin KH
中科院分区：
文献类型：
--
作者：
Cheng WL;Tsai MM;Tsai CY;Huang YH;Chen CY;Chi HC;Tseng YH;Chao IW;Lin WC;Wu SM;Liang Y;Liao CJ;Lin YH;Chung IH;Chen WJ;Lin PY;Wang CS;Lin KH
乙二醇酶I(GLO1)是一种甲基乙醛解毒酶，与人类恶性肿瘤的发生发展密切相关。GLO1在胃癌发生发展中的作用目前尚不清楚。采用定量聚合酶链式反应、免疫组织化学(IHC)和Western blotting方法检测GLO1在胃癌组织中的表达。GLO1在胃癌组织中的表达高于癌旁组织。GLO1的高表达与胃壁侵袭、淋巴结转移和病理分期显著相关，提示GLO1在胃癌的发生发展中起着新的作用。在免疫组化实验中，GLO1低表达组的5年生存率显著高于高表达组(LOG等级P = 0.0373)。GLO1在胃癌细胞系中的过表达增加了细胞的增殖、迁移和侵袭性。相反，用shRNA下调GLO1会导致细胞的迁移和侵袭能力显著降低。我们的研究结果表明，GLO1在胃癌中的高表达增强了肿瘤细胞的体内外转移能力，支持其作为胃癌检测和预后的潜在标志物的有效性。
Glyoxalase I (GLO1), a methylglyoxal detoxification enzyme, is implicated in the progression of human malignancies. The role of GLO1 in gastric cancer development or progression is currently unclear. The expression of GLO1 was determined in primary gastric cancer specimens using quantitative polymerase chain reaction, immunohistochemistry (IHC), and western blotting analyses. GLO1 expression was higher in gastric cancer tissues, compared with that in adjacent noncancerous tissues. Elevated expression of GLO1 was significantly associated with gastric wall invasion, lymph node metastasis, and pathological stage, suggesting a novel role of GLO1 in gastric cancer development and progression. The 5-year survival rate of the lower GLO1 expression groups was significantly greater than that of the higher expression groups (log rank P = 0.0373) in IHC experiments. Over-expression of GLO1 in gastric cancer cell lines increases cell proliferation, migration and invasiveness. Conversely, down-regulation of GLO1 with shRNA led to a marked reduction in the migration and invasion abilities. Our data strongly suggest that high expression of GLO1 in gastric cancer enhances the metastasis ability of tumor cells in vitro and in vivo, and support its efficacy as a potential marker for the detection and prognosis of gastric cancer.
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