Peptide-based vaccinology: experimental and computational approaches to target hypervariable viruses through the fine characterization of protective epitopes recognized by monoclonal antibodies and the identification of T-cell-activating peptides.
Peptide-based vaccinology: experimental and computational approaches to target hypervariable viruses through the fine characterization of protective epitopes recognized by monoclonal antibodies and the identification of T-cell-activating peptides.
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DOI：
10.1155/2013/521231
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发表时间：
2013
影响因子：
--
通讯作者：
Clementi N
中科院分区：
文献类型：
--
作者：
Castelli M;Cappelletti F;Diotti RA;Sautto G;Criscuolo E;Dal Peraro M;Clementi N
确定能够引起保护性免疫反应的病毒蛋白的免疫原域对于开发新的基于表位的预防策略至关重要。这对于选择性靶向高变种病毒共有的保守区尤为重要。研究感染后和免疫后血清，以及克隆和鉴定单抗，仍然是识别保护性表位的最佳途径。特别是，针对保守区的保护性单抗可以在免疫原设计和人类治疗中发挥关键作用。旨在表征保护性mAb表位或识别T细胞激活肽的实验方法往往受到技术限制，可能需要很长时间才能正确解决。因此，在过去的十年里，已经开发了许多表位预测算法。这些算法在不断发展，它们用于解决实证研究的情况也在广泛增加。在这里，我们回顾了几种基于实验技术的策略，这些策略经常用于预测新的基于表位的疫苗方法中包含的免疫原。我们将列出旨在设计一种新的疫苗制剂的主要策略，该制剂具有中和单抗的保护作用，并结合有效的细胞介导的反应。
Defining immunogenic domains of viral proteins capable of eliciting a protective immune response is crucial in the development of novel epitope-based prophylactic strategies. This is particularly important for the selective targeting of conserved regions shared among hypervariable viruses. Studying postinfection and postimmunization sera, as well as cloning and characterization of monoclonal antibodies (mAbs), still represents the best approach to identify protective epitopes. In particular, a protective mAb directed against conserved regions can play a key role in immunogen design and in human therapy as well. Experimental approaches aiming to characterize protective mAb epitopes or to identify T-cell-activating peptides are often burdened by technical limitations and can require long time to be correctly addressed. Thus, in the last decade many epitope predictive algorithms have been developed. These algorithms are continually evolving, and their use to address the empirical research is widely increasing. Here, we review several strategies based on experimental techniques alone or addressed by in silico analysis that are frequently used to predict immunogens to be included in novel epitope-based vaccine approaches. We will list the main strategies aiming to design a new vaccine preparation conferring the protection of a neutralizing mAb combined with an effective cell-mediated response.
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影响因子：
4.2
作者：
Cuccu B;Freer G;Genovesi A;Garzelli C;Rindi L
通讯作者：
Rindi L
影响因子：
3.7
作者：
Burioni, Roberto;Canducci, Filippo;Clementi, Massimo
通讯作者：
Clementi, Massimo
影响因子：
3.7
作者：
Clementi N;De Marco D;Mancini N;Solforosi L;Moreno GJ;Gubareva LV;Mishin V;Di Pietro A;Vicenzi E;Siccardi AG;Clementi M;Burioni R
通讯作者：
Burioni R
影响因子：
2.2
作者：
CZERKINSKY, CC;NILSSON, LA;TARKOWSKI, A
通讯作者：
TARKOWSKI, A
影响因子：
2.7
作者：
El-Manzalawy, Yasser;Dobbs, Drena;Honavar, Vasant
通讯作者：
Honavar, Vasant