The interaction between geminivirus pathogenicity proteins and adenosine kinase leads to increased expression of primary cytokinin-responsive genes.
The interaction between geminivirus pathogenicity proteins and adenosine kinase leads to increased expression of primary cytokinin-responsive genes.
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DOI：
10.1016/j.virol.2010.03.023
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发表时间：
2010-07-05
期刊：
影响因子：
3.7
通讯作者：
Sunter G
中科院分区：
文献类型：
--
作者：
Baliji S;Lacatus G;Sunter G
Begomo病毒和Curto病毒的致病蛋白(AL2/C2)通过抑制腺苷激酶(ADK)而抑制甲基循环，从而抑制沉默。ADK使细胞分裂素核苷磷酸化，通过游离碱、核苷和核苷酸的相互转化，帮助维持生物活性的细胞分裂素库。我们提供的证据表明，抑制ADK会影响主要细胞分裂素反应基因的表达。具体地说，我们证明了在ADK突变的拟南芥植物中，一个主要的细胞分裂素反应启动子的活性增加，并在瞬时试验中对沉默ADK表达或抑制ADK活性做出反应。在双生病毒感染的组织中观察到类似的表达变化，当AL2/C2过度表达时。因此，细胞分裂素反应启动子活性增强可能是ADK/AL2/C2相互作用的结果。外源性细胞分裂素的应用增加了对双生病毒感染的易感性，其特征是平均潜伏期缩短，病毒复制增强。因此，ADK似乎是双生病毒的高价值靶点，包括主要细胞分裂素反应基因的表达增加。
Pathogenicity proteins (AL2/C2) of begomo- and curtoviruses suppress silencing through inhibition of the methyl cycle, as a consequence of inhibiting adenosine kinase (ADK). ADK phosphorylates cytokinin nucleosides, helping maintain a pool of bioactive cytokinins through inter-conversion of free-bases, nucleosides and nucleotides. We provide evidence that inhibiting ADK affects expression of primary cytokinin responsive genes. Specifically, we demonstrate increased activity of a primary cytokinin-responsive promoter in adk mutant Arabidopsis plants, and in response to silencing ADK expression or inhibiting ADK activity in transient assays. Similar changes in expression are observed in geminivirus infected tissue and when AL2/C2 are over-expressed. Increased cytokinin-responsive promoter activity may therefore be a consequence of an ADK/AL2/C2 interaction. Application of exogenous cytokinin increases susceptibility to geminivirus infection, characterized by a reduced mean latent period and enhanced viral replication. Thus, ADK appears to be a high value target of geminiviruses that includes increasing expression of primary cytokinin-responsive genes.
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