Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity.
Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity.
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DOI：
10.1038/s41598-018-32877-2
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发表时间：
2018-10-02
影响因子：
4.6
通讯作者：
Fresno M
中科院分区：
文献类型：
--
作者：
Callejas-Hernández F;Rastrojo A;Poveda C;Gironès N;Fresno M
恰加斯病是一种由原虫锥虫引起的复杂疾病，表现出高度不同的临床结果。从这个意义上讲，基因组序列的阐明和菌株之间的比较可能会导致对疾病的理解。在这里，两个新的克氏锥虫菌株，Y使用Illumina和Bug2148使用PacBio进行了测序，组装、分析并与迄今可用的克氏锥虫注释基因组进行了比较。新序列的组装统计数据表明，克氏锥虫基因组比实际基因组有了有效的改进。例如，在从头尝试中组装的最大重叠群(Bug2148中的1.3 Mb)和最高的平均组装覆盖率(Y的71X)。我们的分析揭示了与感染过程和疾病发展相关的多拷贝基因家族的新的基因组扩张和更大的复杂性，如反唾液酸酶、粘蛋白和粘蛋白相关表面蛋白等。一方面，我们证明了多拷贝基因家族位于由Bug2148组装的类似染色体的1.3 Mb重叠群的端粒区域附近，在那里它们可能承受着很高的进化压力。另一方面，我们鉴定了几个菌株特异性的单拷贝基因，这些基因可能有助于理解菌株之间在感染性和生理学上的差异。综上所述，我们的结果表明，克氏锥虫具有复杂的基因组结构，这可能促进了它的进化。
Chagas disease is a complex illness caused by the protozoan Trypanosoma cruzi displaying highly diverse clinical outcomes. In this sense, the genome sequence elucidation and comparison between strains may lead to disease understanding. Here, two new T. cruzi strains, have been sequenced, Y using Illumina and Bug2148 using PacBio, assembled, analyzed and compared with the T. cruzi annotated genomes available to date. The assembly stats from the new sequences show effective improvement of T. cruzi genome over the actual ones. Such as, the largest contig assembled (1.3 Mb in Bug2148) in de novo attempts and the highest mean assembly coverage (71X for Y). Our analysis reveals a new genomic expansion and greater complexity for those multi-copy gene families related to infection process and disease development, such as Trans-sialidases, Mucins and Mucin Associated Surface Proteins, among others. On one side, we demonstrate that multi-copy gene families are located near telomeric regions of the “chromosome-like” 1.3 Mb contig assembled of Bug2148, where they likely suffer high evolutive pressure. On the other hand, we identified several strain-specific single copy genes that might help to understand the differences in infectivity and physiology among strains. In summary, our results indicate that T. cruzi has a complex genomic architecture that may have promoted its evolution.
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锥虫菌株BUG2148的基因组序列。
DOI：
10.1128/genomea.01497-17
发表时间：
2018-01-18
期刊：
Genome announcements
影响因子：
--
作者：
Callejas-Hernández F;Gironès N;Fresno M
通讯作者：
Fresno M
影响因子：
3.2
作者：
Angel Chiurillo, Miguel;Cortez, Danielle R.;da Silveira, Jose Franco
通讯作者：
da Silveira, Jose Franco
影响因子：
6.7
作者：
Calderon, Jossela;Maganto-Garcia, Elena;Fresno, Manuel
通讯作者：
Fresno, Manuel
影响因子：
4.3
作者：
Cremona, ML;Campetella, O;Frasch, ACC
通讯作者：
Frasch, ACC
影响因子：
14.9
作者：
Bartholomeu, Daniella C.;Cerqueira, Gustavo C.;El-Sayed, Najib M.
通讯作者：
El-Sayed, Najib M.