1H-NMR studies on the gene-5-encoded single-stranded DNA binding protein of the filamentous bacteriophage IKe. General spectral and structural features.
1H-NMR studies on the gene-5-encoded single-stranded DNA binding protein of the filamentous bacteriophage IKe. General spectral and structural features.
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丝状噬菌体 IKe 基因 5 编码的单链 DNA 结合蛋白的 1H-NMR 研究。
DOI：
10.1111/j.1432-1033.1987.tb13373.x
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
C. W. Hilbers
中科院分区：
文献类型：
--
作者：
E. A. de Jong;R. Konings;B. Harmsen;C. W. Prinse;C. W. Hilbers
在生理条件和核磁共振研究所需的浓度下，丝状噬菌体IKe的基因-5蛋白发生严重聚集。描述了可以获得蛋白质的良好分辨率的1H-NMR光谱的条件。利用二维核磁共振技术分析了光谱中的芳香部分；给出了一个完整的解释。寡核苷酸结合研究表明，只有一个苯丙酰残基和一个酪氨酸残基受到rAMP、（dA）2、（dA）3、（dA）4、（dA）6、d(pT)3或（dT）4结合的影响。结合后，这些氨基酸残基的芳香共振向上移动约0.4-0.5 ppm。在不同pH值下的核磁共振测量表明，两个组氨酸残基中只有一个是可自由滴定的。从CIDNP实验中得出结论，五个酪氨酸残基中有三个位于蛋白质表面。作为蛋白质浓度函数进行的测量表明，二聚体基因5-蛋白质分子之间发生了特定的蛋白质-蛋白质相互作用。所得数据与M13基因5蛋白的现有数据进行了比较。从比较中可以看出，这些蛋白质几乎在各个方面都是相互模仿的。
Under physiological conditions and at concentrations needed for NMR studies, severe aggregation of the gene-5 protein of the filamentous phage IKe occurs. Conditions are described for which well-resolved 1H-NMR spectra of the protein can be obtained. The aromatic part of the spectrum is analyzed by means of two-dimensional NMR techniques; a complete interpretation is presented. Oligonucleotide binding studies reveal that just one phenylalanyl residue and one tyrosyl residue are influenced by the binding of rAMP, (dA)2, (dA)3, (dA)4, (dA)6, d(pT)3 or (dT)4. Upon binding, the aromatic resonances of these amino acid residues are shifted upfield by about 0.4-0.5 ppm. NMR measurements at different pH values demonstrate that only one of the two histidyl residues is freely titratable. From CIDNP experiments it is concluded that three out of five tyrosyl residues are located at the surface of the protein. Measurements carried out as a function of protein concentration indicate the occurrence of specific protein-protein interactions between dimeric gene-5-protein molecules. The data obtained are compared with those available for the gene-5 protein of M13. It follows from the comparison that these proteins mimic each other in almost every respect.
fd 基因 5 DNA 结合蛋白中赖氨酰残基的还原甲基化：修饰蛋白的 CD 和 13C NMR 结果。
影响因子：
4.4
作者：
Gray,DM;Sherry,AD;Teherani,J;Kansy,JW
通讯作者：
Kansy,JW