Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain.
Effect of Massage on the TLR4 Signalling Pathway in Rats with Neuropathic Pain.
复制标题
按摩对神经病理性疼痛大鼠TLR4信号通路的影响
DOI：
10.1155/2020/8309745
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发表时间：
2020
影响因子：
2.9
通讯作者：
Tang H
中科院分区：
文献类型：
--
作者：
Wang Q;Lin J;Yang P;Liang Y;Lu D;Wang K;Gan W;Fu J;Gan Z;Ma M;Wu P;He F;Pang J;Tang H
本研究旨在探讨按摩对脊神经结扎（SNL）大鼠背根神经节中Toll样受体4（TLR 4）信号通路的影响，并假设按摩可用作镇痛剂。将40只雌性SD大鼠随机分为5组：对照组、假手术组、模型组、假按摩组、按摩组。每组8只大鼠。模型组、假推拿组、推拿组建立SNL大鼠模型。假手术组大鼠接受手术暴露椎神经，但不进行进一步的操作。对照组由完整动物组成。按摩组采用按摩模拟机进行按摩，每天1次，连续14 d;假按摩组采用布袋轻轻触摸大鼠后肢，每天1次，连续14 d。对照组、假手术组、模型组不作任何干预，观察14 d。分别于造模前1 d、造模后1、3、7、14 d检测各组大鼠缩足热潜伏期（PWTL）和缩足机械阈值（PWMT）。造模后14 d，检测各组大鼠TLR 4、IRAK 1、TRAF 6、TNF-α、IL-6的表达水平。推拿后SNL大鼠PWTL、PWMT降低，而PWTL、PWMT升高。SNL大鼠的TLR 4、IRAK 1、TRAF 6、IL-6和TNF-α水平较高，按摩可有效降低这些分子的表达水平。抑制TLR 4信号通路的激活，可以减少炎症因子的释放，可能是按摩治疗神经性疼痛的一种机制。
This study set out to investigate the effect of massage on the Toll-like receptor 4 (TLR4) signalling pathway in the dorsal root ganglia of rats that had undergone spinal nerve ligation (SNL), with the hypothesis that massage could be used as an analgesic. Forty female SD rats were randomly divided into 5 groups: the control group, sham-operated group, model group, sham massage group, and massage group. There were 8 rats in each group. SNL rat models were established in the model group, sham massage group, and massage group. Rats in the sham-operated group underwent surgery to expose the vertebral nerves, but no further procedures were performed. The control group consisted of intact animals. The rats in the massage group underwent massage using a massage simulation machine once a day for 14 d in succession; the hind limbs of the rats in the sham massage group were gently touched with a cloth bag once a day for 14 continuous days. The rats in the control group, the sham-operated group, and the model group did not receive any intervention and were observed for 14 d. Paw withdrawal thermal latency (PWTL) and paw withdrawal mechanical threshold (PWMT) of rats in each group were detected 1 d before modelling and at 1, 3, 7, and 14 d after modelling. Fourteen days after modelling, the expression levels of TLR4, IRAK1, TRAF6, TNF-α, and IL-6 were detected in all rats. The PWTL and PWMT of SNL rats were decreased, while these parameters were elevated after massage. SNL rats showed higher levels of TLR4, IRAK1, TRAF6, IL-6, and TNF-α, and massage effectively lowered the expression levels of these molecules. Inhibiting activation of the TLR4 signalling pathway, which can reduce the release of inflammatory factors, may be one mechanism by which massage treats neuropathic pain.
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影响因子：
56.9
作者：
MELZACK, R;WALL, PD
通讯作者：
WALL, PD
神经炎症驱动的慢性疼痛的新兴目标。
影响因子：
120.1
作者：
Ji, Ru-Rong;Xu, Zhen-Zhong;Gao, Yong-Jing
通讯作者：
Gao, Yong-Jing
影响因子：
4
作者：
Gaskin, Darrell J.;Richard, Patrick
通讯作者：
Richard, Patrick
影响因子：
5.6
作者：
Ma, Bo;Meng, Xiangbao;Sun, Xiaobo
通讯作者：
Sun, Xiaobo
DOI：
10.1007/s00018-014-1762-5
发表时间：
2015-02
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Płóciennikowska A;Hromada-Judycka A;Borzęcka K;Kwiatkowska K
通讯作者：
Kwiatkowska K