von Willebrand factor release and thrombomodulin and tissue factor expression in Rickettsia conorii-infected endothelial cells
von Willebrand factor release and thrombomodulin and tissue factor expression in Rickettsia conorii-infected endothelial cells
复制标题
康氏立克次体感染的内皮细胞中冯维勒布兰德因子的释放以及血栓调节蛋白和组织因子的表达
DOI：
10.1128/iai.60.10.4388-4393.1992
复制
发表时间：
1992
影响因子：
3.1
通讯作者：
D. Raoult
中科院分区：
文献类型：
--
作者：
N. Teysseire;D. Arnoux;F. George;J. Sampol;D. Raoult
Mediterranean spotted fever, a tick-borne rickettsiosis caused by Rickettsia conorii, may lead to small-vessel or deep-vein thrombosis. In order to evaluate the role of endothelial cell alteration in this lesion, we infected human endothelial cells derived from umbilical veins with R. conorii. We report the induction of two previously unreported prothrombotic mechanisms in rickettsial disease: (i) a progressive decline in thrombomodulin antigen and (ii) early expression of tissue factor, and, as described for R. rickettsii infection, later release of von Willebrand factor from Weibel-Palade bodies. Thrombomodulin expression in infected endothelial cells, measured by the thrombin-dependent activation of protein C or flow cytometric analysis, decreased steadily between 4 and 24 h after inoculation with rickettsiae. R. conorii infection induced tissue factor expression, measured by clotting assay and flow cytometric analysis, which was detectable 2 h postinoculation, reached its maximum 4 h postinoculation, and progressively decreased thereafter. Infection resulted in a relatively late release of von Willebrand factor antigen into the culture medium. A double-label immunofluorescence assay for the simultaneous evaluation of von Willebrand factor and R. conorii showed that the depletion of cytoplasmic von Willebrand factor stored in Weibel-Palade bodies was due to a direct effect of the intracellular R. conorii. These disturbances of endothelial function observed with R. conorii-infected cells may provide a paradigm for the elucidation of thrombotic pathobiology with Mediterranean spotted fever.
登录
查看更多内容
内皮前列腺素分泌：斑疹伤寒立克次体的影响。
DOI：
10.1093/infdis/162.5.1136
发表时间：
1990
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Walker,TS;Brown,JS;Hoover,CS;Morgan,DA
通讯作者：
Morgan,DA
内皮止血特性的调节：在宿主反应中发挥积极作用。
影响因子：
10.5
作者：
Gerlach,H;Esposito,C;Stern,DM
通讯作者：
Stern,DM
落基山斑疹热早期阶段血小板和血浆蛋白水解系统的前瞻性研究。
DOI：
10.1056/nejm198804213181603
发表时间：
1988
期刊：
The New England journal of medicine
影响因子：
--
作者：
Rao,AK;Schapira,M;Clements,ML;Niewiarowski,S;Budzynski,AZ;Schmaier,AH;Harpel,PC;Blackwelder,WC;Scherrer,JR;Sobel,E
通讯作者：
Sobel,E
立克次氏体对人血管内皮细胞的感染。
DOI：
10.1093/infdis/149.2.201
发表时间：
1984
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Silverman,DJ;Bond,SB
通讯作者：
Bond,SB
地中海斑疹热引起的急性血管损伤期间蛋白 C 通路激活的证据。
影响因子：
20.3
作者：
Vicente,V;España,F;Tabernero,D;Estellés,A;Aznar,J;Hendl,S;Griffin,JH
通讯作者：
Griffin,JH