Angiogenic activity of bovine corpora lutea at several stages of luteal development.
Angiogenic activity of bovine corpora lutea at several stages of luteal development.
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牛黄体在黄体发育的几个阶段的血管生成活性。
DOI：
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
L. Reynolds
中科院分区：
文献类型：
--
作者：
D. Redmer;A. Grazul;J. Kirsch;L. Reynolds
测定了出血体、中期黄体和晚期黄体对发育雏鸡绒毛尿囊膜新生血管的刺激作用。回答从0分到4分(4分为最高分)。发情周期各阶段的黄体组织植入刺激了CAM血管的生长，血管反应随着CL年龄的增加而增加。不同发育阶段的黄体组织在不含激素、促黄体生成素和/或前列腺素F-2α的无血清培养液中孵育6小时。测定黄体组织条件培养液中孕酮的含量，并检测黄体组织条件培养液对体外培养的牛主动脉内皮细胞有丝分裂和迁移的刺激作用。所有的黄体组织条件培养液都刺激内皮细胞有丝分裂和迁移，但来自周期晚期CL的培养液表现出最大的活性。促黄体生成素在体外显著增加一种刺激内皮细胞迁移的因子(S)的分泌。单独前列腺素F-2α对黄体孵育的内皮细胞有丝分裂原或迁移刺激因子(S)的产生无影响；但促黄体生成素促进迁移刺激因子(S)分泌的能力被前列腺素F-2α阻断。这项研究表明，牛黄体组织的血管生成活性随黄体年龄的增加而增加，体外血管生成因子的分泌对已知的调节黄体功能的激素有反应。
Samples from corpus haemorrhagicum, mid-cycle corpus luteum (CL) and late-cycle CL were tested for their abilities to stimulate neovascularization of chorioallantoic membranes (CAM) of developing chicks. Responses were graded from 0 to 4 (4 being the greatest response). Luteal tissue implants from each stage of the oestrous cycle stimulated growth of CAM blood vessels, and vascular responses increased with age of CL. Implants from late-cycle CL were typically graded 3 or 4. Luteal tissues from several stages of development were also incubated for 6 h in serum-free medium containing no hormone, LH, PGF-2 alpha or both hormones. Media conditioned by luteal tissues were assayed for progesterone and tested for their ability to stimulate mitogenesis and migration of bovine aortic endothelial cells in vitro. All media conditioned by luteal tissues stimulated mitogenesis and migration of endothelial cells, but media from late-cycle CL exhibited the greatest activity. Luteinizing hormone significantly increased in-vitro secretion of a factor(s) that stimulated migration of endothelial cells. PGF-2 alpha alone had no effect on production of endothelial cell mitogen or migration-stimulating factor(s) from luteal incubations; however, the ability of LH to enhance secretion of the migration-stimulating factor(s) was blocked by PGF-2 alpha. This study demonstrates that angiogenic activity of bovine luteal tissues increases with age of the CL and in-vitro secretion of angiogenic factor is responsive to hormones known to regulate luteal function.
大鼠颗粒细胞条件培养基刺激内皮细胞增殖。
影响因子：
4.8
作者：
Koos,RD
通讯作者：
Koos,RD
影响因子：
56.9
作者：
FOLKMAN, J;LANGER, R;TAYLOR, S
通讯作者：
TAYLOR, S