Regulation of mouse oocyte microtubule and organelle dynamics by PADI6 and the cytoplasmic lattices.
Regulation of mouse oocyte microtubule and organelle dynamics by PADI6 and the cytoplasmic lattices.
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DOI：
10.1016/j.ydbio.2010.11.033
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发表时间：
2011-02-15
影响因子：
2.7
通讯作者：
Coonrod SA
中科院分区：
文献类型：
--
作者：
Kan R;Yurttas P;Kim B;Jin M;Wo L;Lee B;Gosden R;Coonrod SA
卵母细胞中细胞器的定位和运动在很大程度上是由微管及其相关的运动蛋白调节的。虽然尚未在生殖细胞中进行研究，但马达蛋白对乙酰化MTS的特异性识别也促进了体细胞中的货物运输。我们之前已经证明，限制卵母细胞的PADI6对于形成一种新的限制卵母细胞的纤维结构--细胞质晶格(CPL)是必不可少的。在这里，我们发现α-微管蛋白似乎与PADI6/CPL复合体有关。接下来，我们证明了在PADI6缺失的卵母细胞中细胞器的定位和重新分布是有缺陷的，MT聚合或MT马达活性的改变不会在这些卵母细胞中引起细胞器的重新分布。最后，我们报道了在PADI6缺失的卵母细胞中，细胞质中乙酰化微管的水平被显著抑制，这表明观察到的细胞器重新分布失败是由于稳定的细胞质MT的缺陷所致。这些结果表明，PADI6/CPL超结构在调节MT介导的细胞器定位和运动中起着关键作用。
Organelle positioning and movement in oocytes is largely mediated by microtubules (MTs) and their associated motor proteins. While yet to be studied in germ cells, cargo trafficking in somatic cells is also facilitated by specific recognition of acetylated MTs by motor proteins. We have previously shown that oocyte-restricted PADI6 is essential for formation of a novel oocyte-restricted fibrous structure, the cytoplasmic lattices (CPLs). Here, we show that α-tubulin appears to be associated with the PADI6/CPL complex. Next, we demonstrate that organelle positioning and redistribution is defective in PADI6-null oocytes and that alteration of MT polymerization or MT motor activity does not induce organelle redistribution in these oocytes. Finally, we report that levels of acetylated microtubules are dramatically suppressed in the cytoplasm of PADI6-null oocytes, suggesting that the observed organelle redistribution failure is due to defects in stable cytoplasmic MTs. These results demonstrate that the PADI6/CPL superstructure plays a key role in regulating MT-mediated organelle positioning and movement.
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影响因子：
64.8
作者：
Hubbert, C;Guardiola, A;Yao, TP
通讯作者：
Yao, TP
DOI：
10.1083/jcb.200911024
发表时间：
2010-08-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Friedman JR;Webster BM;Mastronarde DN;Verhey KJ;Voeltz GK
通讯作者：
Voeltz GK
影响因子：
3.7
作者：
Kim B;Kan R;Anguish L;Nelson LM;Coonrod SA
通讯作者：
Coonrod SA
影响因子：
2.5
作者：
Lessman, CA;Kim, H
通讯作者：
Kim, H
影响因子：
2.7
作者：
Combelles, CMH;Albertini, DF
通讯作者：
Albertini, DF