Potential role for MATER in cytoplasmic lattice formation in murine oocytes.
Potential role for MATER in cytoplasmic lattice formation in murine oocytes.
复制标题
DOI：
10.1371/journal.pone.0012587
复制
发表时间：
2010-09-07
期刊：
影响因子：
3.7
通讯作者：
Coonrod SA
中科院分区：
文献类型：
--
作者：
Kim B;Kan R;Anguish L;Nelson LM;Coonrod SA
Mater和Padi6是母系效应基因，在卵母细胞生长过程中首次表达，在小鼠的双细胞阶段以后的胚胎发育中是必需的。我们最近发现PADI6定位于丰富的纤维状Triton X-100 （Triton）不溶性结构（称为卵母细胞细胞质晶格（cpl））的形成，并且是形成所需的。鉴于它们相似的表达谱和突变小鼠表型，我们一直在测试MATER也在CPL形成和/或功能中起作用的假设。本研究表明，在Triton提取后，PADI6和MATER在整个卵母细胞细胞质中共定位，表明MATER在cpl处与PADI6共定位。此外，提取Triton后，PADI6在Matertm/tm卵母细胞中的溶解度显著增加，表明MATER参与了CPL成核。这一预测得到了Mater+/+和Matertm/tm生发囊期卵母细胞透射电镜分析的支持，该分析表明，与Mater+/+卵母细胞相比，Matertm/tm卵母细胞中cpl的体积分数减少了90%。综上所述，这些结果表明，与PADI6类似，MATER也是CPL形成所必需的。鉴于PADI6和MATER对女性生育至关重要，这些结果不仅加强了晶格在卵母细胞向胚胎转变过程中起关键作用的假设，而且增加了我们对cpl分子性质的理解。
Mater and Padi6 are maternal effect genes that are first expressed during oocyte growth and are required for embryonic development beyond the two-cell stage in the mouse. We have recently found that PADI6 localizes to, and is required for the formation of, abundant fibrillar Triton X-100 (Triton) insoluble structures termed the oocyte cytoplasmic lattices (CPLs). Given their similar expression profiles and mutant mouse phenotypes, we have been testing the hypothesis that MATER also plays a role in CPL formation and/or function. Herein, we show that PADI6 and MATER co-localize throughout the oocyte cytoplasm following Triton extraction, suggesting that MATER co-localizes with PADI6 at the CPLs. Additionally, the solubility of PADI6 was dramatically increased in Matertm/tm oocytes following Triton extraction, suggesting that MATER is involved in CPL nucleation. This prediction is supported by transmission electron microscopic analysis of Mater+/+ and Matertm/tm germinal vesicle stage oocytes which illustrated that volume fraction of CPLs was reduced by 90% in Matertm/tm oocytes compared to Mater+/+ oocytes. Taken together, these results suggest that, similar to PADI6, MATER is also required for CPL formation. Given that PADI6 and MATER are essential for female fertility, these results not only strengthen the hypothesis that the lattices play a critical role in mediating events during the oocyte-to-embryo transition but also increase our understanding of the molecular nature of the CPLs.
登录
查看更多内容
卵巢基因表达在没有图形的情况下，卵母细胞特异性转录因子。
影响因子：
--
作者：
Joshi, Saurabh;Davies, Holly;Sims, Lauren Porter;Levy, Shawn E.;Dean, Jurrien
通讯作者：
Dean, Jurrien
影响因子：
37.3
作者：
Gorin MA;Pan Q
通讯作者：
Pan Q
影响因子：
4.8
作者：
Tong, ZB;Gold, L;Nelson, LM
通讯作者：
Nelson, LM
影响因子：
4.8
作者：
Tong, ZB;Nelson, LM
通讯作者：
Nelson, LM
影响因子：
3.4
作者：
Niimura, Sueo;Kawakami, Shin-Ya;Takano, Hiroko
通讯作者：
Takano, Hiroko