Prevalence of genotoxic bacteria in men undergoing biopsy for prostate cancer.
Prevalence of genotoxic bacteria in men undergoing biopsy for prostate cancer.
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DOI：
10.1002/pros.24500
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发表时间：
2023-05
期刊：
影响因子：
2.8
通讯作者：
Liss, Michael A.
中科院分区：
文献类型：
--
作者：
Lee, John;Wickes, Brian L.;Fu, Jianmin;Brockman, Nohelli E.;Garg, Harshit;Jobin, Christian;Johson-Pais, Teresa;Leach, Robin;Lai, Zhao;Liss, Michael A.
新的证据表明，细菌产生的DNA毒素可能在前列腺癌的发展或进展中起作用。为了确定这些基因在非感染（即，殖民）状态下，我们在进行前列腺癌检测的活组织检查之前筛选男性的尿液样本。我们开发了一种多重聚合酶链反应，使用三种最常见的细菌遗传毒素基因引物：Colibactin（聚酮合酶[pks]基因岛：clbN和clb B），细胞毒性坏死因子（cnf 1）毒素和细胞致死膨胀毒素B（cdt B）代表基因岛。在对已知基因型的大肠杆菌样品进行校准后，我们使用了训练和验证队列。我们对45名接受前列腺活检的男性先前收集的尿液进行了多重检测。对于验证队列，我们使用了先前随机临床试验（n = 263）中已知前列腺癌结局的基线尿液样本。前列腺癌患者前列腺活检前尿液中检出4种常见细菌遗传毒素基因的检出率为8%（25/311）。pks岛（clbN和clbB）、cnf 1和cdt毒素基因的检出率分别为6.1%、2.4%和1.7%。我们发现尿基因毒素和前列腺癌之间没有关联（p = 0.83）。我们确实发现，在尿基因毒素阳性的男性中，低级别癌症的比例较高（92%对44%），而在基因毒素阴性的男性中，高级别癌症的比例较高（8%对56%，p = 0.001）。尿基因毒素的患病率很低，与前列腺癌诊断不一致。尿液是在一个时间点采集的，不排除既往暴露的可能性。
New evidence suggests that bacteria-produced DNA toxins may have a role in the development or progression of prostate cancer. To determine the prevalence of these genes in a noninfection (i.e., colonized) state, we screened urine specimens in men before undergoing a biopsy for prostate cancer detection. We developed a multiplex polymerase chain reaction using three of the most described bacterial genotoxin gene primers: Colibactin (polyketone synthase [pks] gene island: clbN and clbB), cytotoxic necrotizing factor (cnf1) toxin, and cytolethal distending toxin B (cdtB) represented gene islands. After calibration on Escherichia coli samples of known genotypes, we used a training and validation cohort. We performed multiplex testing on a training cohort of previously collected urine from 45 men undergoing prostate biopsy. For the validation cohort, we utilized baseline urine samples from a previous randomized clinical trial (n = 263) with known prostate cancer outcomes. The prevalence of four common bacterial genotoxin genes detected in the urine before prostate biopsy for prostate cancer is 8% (25/311). The prevalence of pks island (clbN and clbB), cnf1, and cdt toxin genes are 6.1%, 2.4%, and 1.7%, respectively. We found no association between urinary genotoxins and prostate cancer (p = 0.83). We did identify a higher proportion of low-grade cancer (92% vs. 44%) in those men positive for urinary genotoxin and higher-grade cancer in those genotoxin negative (8% vs. 56%, p = 0.001). The prevalence of urinary genotoxins is low and does not correspond to a prostate cancer diagnosis. The urine was taken at one point in time and does not rule out the possibility of previous exposure.
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影响因子：
5.7
作者：
Graillot, Vanessa;Dormoy, Inge;Vignard, Julien
通讯作者：
Vignard, Julien
影响因子：
56.9
作者：
Nougayrede, Jean-Philippe;Homburg, Stefan;Oswald, Eric
通讯作者：
Oswald, Eric
DOI：
10.1073/pnas.2018976118
发表时间：
2021-08-10
影响因子：
11.1
作者：
Shrestha, Eva;Coulter, Jonathan B.;Sfanos, Karen S.
通讯作者：
Sfanos, Karen S.
影响因子：
9.4
作者：
Johnson, James R.;Johnston, Brian D.;Gordon, David M.
通讯作者：
Gordon, David M.
影响因子：
56.9
作者：
Xue, Mengzhao;Kim, Chung Sub;Crawford, Jason M.
通讯作者：
Crawford, Jason M.