Which fluorophore is brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, texas red, and cyanine 3.18
Which fluorophore is brightest? A comparison of the staining obtained using fluorescein, tetramethylrhodamine, lissamine rhodamine, texas red, and cyanine 3.18
复制标题
哪种荧光团最亮？
DOI：
10.1007/bf00716998
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
T. Brelje
中科院分区：
文献类型：
--
作者：
M. Wessendorf;T. Brelje
有几种红色荧光团可用于免疫荧光研究，包括四甲基罗丹明，丽丝胺罗丹明，德克萨斯红和花青3.18;然而，目前还不清楚哪种是最好的。本研究将这些荧光团的亮度与荧光素的亮度进行比较。尝试使用针对5-羟色胺产生的一抗和与荧光素或红色荧光团之一缀合的二抗进行染色。通过光密度测定法确定染色强度。发现花青3.18的缀合物提供比任何其它荧光团（包括荧光素）的缀合物显著更亮的染色。它的结论是，花青3.18应该是有用的荧光实验，它可能是最亮的荧光团可用于单色荧光免疫细胞化学。
SummaryThere are several red-emitting fluorophores available for immunofluorescence studies, including tetramethylrhodamine, lissamine rhodamine, Texas Red, and cyanine 3.18; however, it is unclear which of these is best. The present study compared the brightness of these fluorophores to that of fluorescein. Staining was attempted using a primary antibody raised against serotonin and a secondary antibody that was conjugated with either fluorescein or one of the red fluorophores. The intensity of staining was determined densitometrically. It was found that a conjugate of cyanine 3.18 provided significantly brighter staining that conjugates of any of the other fluorophores, including fluorescein. It is concluded that cyanine 3.18 should be useful for multicolor fluorescence experiments and that it may be the brightest fluorophore available for single-color fluorescence immunocytochemistry.
影响因子：
4.7
作者：
MUJUMDAR, RB;ERNST, LA;WAGGONER, AS
通讯作者：
WAGGONER, AS
一种使用荧光团荧光素、丽丝胺罗丹明和 7-氨基-4-甲基香豆素-3-乙酸对大鼠大脑和脊髓中三种共存神经递质进行免疫荧光演示的方法。
DOI：
10.1177/38.12.1701460
发表时间：
1990
期刊：
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
影响因子：
--
作者：
Wessendorf,MW;Appel,NM;Molitor,TW;Elde,RP
通讯作者：
Elde,RP