Hyperion Image Analysis Depicts a Preliminary Landscape of Tumor Immune Microenvironment in OSCC with Lymph Node Metastasis.
Hyperion Image Analysis Depicts a Preliminary Landscape of Tumor Immune Microenvironment in OSCC with Lymph Node Metastasis.
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Hyperion 图像分析描绘了伴有淋巴结转移的 OSCC 肿瘤免疫微环境的初步景观
DOI：
10.1155/2021/9975423
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发表时间：
2021
影响因子：
4.1
通讯作者：
Cai ZG
中科院分区：
文献类型：
--
作者：
Xie S;Zhang XY;Shan XF;Yau V;Zhang JY;Wang W;Yan YP;Cai ZG
背景口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）是口腔癌中最常见的类型。由于其预后差异很大，识别肿瘤免疫微环境可能是治疗计划和预测更准确预后的关键工具。本研究的目的是利用免疫成像系统来描绘口腔鳞癌淋巴结转移的肿瘤免疫微环境的初步景观。方法收集口腔鳞癌患者的肿瘤标本。获得其福尔马林固定、石蜡包埋（FFPE）组织切片，并使用一组26种临床相关金属偶联抗体进行染色。用荧光显微镜成像系统对这些染色的细胞进行检测和分析。结果4例患者符合入选标准。我们描绘了他们的肿瘤免疫微环境的初步景观，并根据表型相似性从这些OSCC患者中鉴定了25种不同的免疫细胞亚群。所有这些患者的肿瘤标本中的CD8+ T细胞表达均降低。细胞亚群的多样性，患者A和患者B的免疫活化细胞多于患者C和患者D。肿瘤免疫微环境的这种差异有助于预测个体肿瘤。结论口腔鳞癌淋巴结转移患者的免疫微环境可初步、多方位地反映口腔鳞癌淋巴结转移的免疫微环境特征，有助于了解免疫微环境对口腔鳞癌淋巴结转移的影响。这些结果揭示了口腔鳞状细胞癌淋巴结转移患者不同预后的可能影响因素，为制定个体化治疗方案提供参考。
Background Oral squamous cell carcinoma (OSCC) constitutes the most common types of oral cancer. Because its prognosis varies significantly, identification of a tumor immune microenvironment could be a critical tool for treatment planning and predicting a more accurate prognosis. This study is aimed at utilizing the Hyperion imaging system to depict a preliminary landscape of the tumor immune microenvironment in OSCC with lymph node metastasis. Methods We collected neoplasm samples from OSCC patients. Their formalin-fixed, paraffin-embedded (FFPE) tissue sections were obtained and stained utilizing a panel of 26 clinically relevant metal-conjugated antibodies. Detection and analysis were performed for these stained cells with the Hyperion imaging system. Results Four patients met our inclusion criteria. We depicted a preliminary landscape of their tumor immune microenvironment and identified 25 distinct immune cell subsets from these OSCC patients based on phenotypic similarity. All these patients had decreased expression of CD8+ T cells in tumor specimens. Variety in cell subsets was seen, and more immune activated cells were found in patient A and patient B than those in patient C and patient D. Such differences in tumor immune microenvironments can contribute to forecasting of individual prognoses. Conclusion The Hyperion imaging system helped to delineate a preliminary and multidimensional landscape of the tumor immune microenvironment in OSCC with lymph node metastasis and provided insights into the influence of the immune microenvironment in determination of prognoses. These results reveal possible contributory factors behind different prognoses of OSCC patients with lymph node metastasis and provide reference for individual treatment planning.
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影响因子：
64.5
作者：
Levine JH;Simonds EF;Bendall SC;Davis KL;Amir el-AD;Tadmor MD;Litvin O;Fienberg HG;Jager A;Zunder ER;Finck R;Gedman AL;Radtke I;Downing JR;Pe'er D;Nolan GP
通讯作者：
Nolan GP
影响因子：
5.2
作者：
Richardson JR;Schöllhorn A;Gouttefangeas C;Schuhmacher J
通讯作者：
Schuhmacher J
影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
DOI：
10.1007/s00018-017-2686-7
发表时间：
2018-03
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Ostroumov D;Fekete-Drimusz N;Saborowski M;Kühnel F;Woller N
通讯作者：
Woller N
影响因子：
48
作者：
Korsunsky, Ilya;Millard, Nghia;Raychaudhuri, Soumya
通讯作者：
Raychaudhuri, Soumya