Serum MicroRNA Levels as a Noninvasive Diagnostic Biomarker for the Early Diagnosis of Hepatitis B Virus-Related Liver Fibrosis.
Serum MicroRNA Levels as a Noninvasive Diagnostic Biomarker for the Early Diagnosis of Hepatitis B Virus-Related Liver Fibrosis.
复制标题
血清 MicroRNA 水平作为乙型肝炎病毒相关肝纤维化早期诊断的无创诊断生物标志物
DOI：
10.5009/gnl16560
复制
发表时间：
2017-11-15
期刊：
影响因子：
3.4
通讯作者：
Shi G
中科院分区：
文献类型：
--
作者：
Bao S;Zheng J;Li N;Huang C;Chen M;Cheng Q;Yu K;Chen S;Zhu M;Shi G
目的：探讨血清微核糖核酸(MiRNA)水平作为鉴别慢性乙型肝炎患者S0-S2(早期纤维化)和S3-S4(晚期纤维化)的潜在无创性生物标志物的作用。123名接受肝活检的慢性乙肝病毒感染的未接受治疗的患者参加了这项研究。用实时定量聚合酶链式反应检测选定的miRNAs水平。采用Logistic回归分析评估与纤维化进展相关的因素。受试者工作特征(ROC)曲线和判别分析验证了这些预测变量区分S0-S2和S3-S4的能力。随着纤维化从S0-S2发展到S3-S4，血清miR-29、miR-143、miR-223、miR-21和miR-374水平显著降低(p<；0.05)，但miR-16水平无明显变化。多元Logistic回归分析确定了三组miRNA和血小板，它们在区分S0-S2和S3-S4方面具有很高的诊断准确性，曲线下的面积为0.936。除miR-16外，所研究的miRNAs水平随纤维化进展而变化。一个专家小组被确定能够区分S0-S2和S3-S4，表明血清miRNA水平可以作为潜在的非侵入性纤维化进展的生物标志物。
To investigate the role of selected serum microRNA (miRNA) levels as potential noninvasive biomarkers for differentiating S0–S2 (early fibrosis) from S3–S4 (late fibrosis) in patients with a chronic hepatitis B virus (HBV) infection. One hundred twenty-three treatment-naive patients with a chronic HBV infection who underwent a liver biopsy were enrolled in this study. The levels of selected miRNAs were measured using a real-time quantitative polymerase chain reaction assay. A logistic regression analysis was performed to assess factors associated with fibrosis progression. Receiver operating characteristic (ROC) curve and discriminant analyses validated these the ability of these predicted variables to discriminate S0–S2 from S3–S4. Serum miR-29, miR-143, miR-223, miR-21, and miR-374 levels were significantly downregulated as fibrosis progressed from S0–S2 to S3–S4 (p<0.05), but not miR-16. The multivariate logistic regression analysis identified a panel of three miRNAs and platelets that were associated with a high diagnostic accuracy in discriminating S0–S2 from S3–S4, with an area under the curve of 0.936. The levels of the studied miRNAs, with the exception of miR-16, varied with fibrosis progression. A panel was identified that was capable of discriminating S0–S2 from S3–S4, indicating that serum miRNA levels could serve as a potential noninvasive biomarker of fibrosis progression.
登录
查看更多内容
影响因子：
5.7
作者：
Naito Y;Sakamoto N;Oue N;Yashiro M;Sentani K;Yanagihara K;Hirakawa K;Yasui W
通讯作者：
Yasui W
影响因子：
3.2
作者：
Liu, Xiujuan;Hong, Quan;Xu, Lihong
通讯作者：
Xu, Lihong
影响因子：
64.8
作者：
Davis, Brandi N.;Hilyard, Aaron C.;Hata, Akiko
通讯作者：
Hata, Akiko
影响因子：
13.5
作者：
DESMET, VJ;GERBER, M;SCHEUER, PJ
通讯作者：
SCHEUER, PJ
影响因子：
25.7
作者：
Trebicka, Jonel;Anadol, Evrim;Odenthal, Margarete
通讯作者：
Odenthal, Margarete