Robust marking of photoreceptor cells and pinealocytes with several reporters under control of the Crx gene.
Robust marking of photoreceptor cells and pinealocytes with several reporters under control of the Crx gene.
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DOI：
10.1002/dvdy.22138
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发表时间：
2009-12
影响因子：
2.5
通讯作者：
Cepko, Constance L.
中科院分区：
文献类型：
--
作者：
Samson, Maria;Emerson, Mark M.;Cepko, Constance L.
Crx是同源异型盒基因Otx家族的成员，其表达限于脊椎动物视网膜感光细胞和双极细胞以及松果体器官的松果体细胞。为了促进Crx表达细胞的可视化，我们产生了在细菌人工染色体（BAC）内存在的Crx调控序列的控制下表达几种报告基因的转基因小鼠。这些小鼠通过提供更广泛的报告基因库，扩大了转基因小鼠的收集，其使用光感受器调节元件进行报告基因表达。此外，由于Crx在注定成为感光细胞的细胞变成有丝分裂后很快表达，它们提供了早期鉴定未成熟感光细胞的手段。
Crx is a member of the Otx family of homeobox genes with expression restricted to vertebrate retinal photoreceptor and bipolar cells as well as the pinealocytes of the pineal organ. To facilitate the visualization of Crx-expressing cells, we generated transgenic mice expressing several reporters under the control of the Crx regulatory sequences present within a bacterial artificial chromosome (BAC). These mice expand the transgenic mouse collection, which uses photoreceptor regulatory elements for reporter gene expression by providing a broader repertoire of reporter genes. In addition, since Crx is expressed very soon after a cell fated to be a photoreceptor cell becomes postmitotic, they provide a means for early identification of immature photoreceptor cells.
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