Murine mentors: transgenic and knockout models of surgical disease.
Murine mentors: transgenic and knockout models of surgical disease.
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小鼠导师：外科疾病的转基因和敲除模型。
DOI：
10.1097/00000658-199901000-00004
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发表时间：
1999
影响因子：
9
通讯作者：
Hirose,R
中科院分区：
文献类型：
--
作者：
Arbeit,JM;Hirose,R
目的 转基因和基因敲除技术已经从“分子生物学革命”中出现，成为操纵完整小鼠基因功能的前所未有的技术。本综述的目的是概述创建转基因和基因敲除小鼠的技术，并证明它们在阐明常见外科疾病的分子机制中的用途。背景数据摘要基因功能的获得是通过转基因技术实现的，而基因功能是通过基因敲除来消除的。每种技术都有独特的应用和缺点。基因操纵小鼠的一个独特特征是可以进行组合基因实验，精确定义基因对疾病进展的功能贡献。伤口愈合、心血管疾病、移植免疫学、肠道运动和炎症性肠病以及肿瘤学的转基因和基因敲除小鼠模型开始阐明这些疾病的精确分子调控。转基因技术也已扩展到猪等大型哺乳动物，其目标是利用异种免疫反应的基因操作来增加移植器官的可用性。小鼠基因操纵技术的不断完善提供了根据研究人员的需要以精确的时间间隔和特定组织打开或关闭基因的机会。最终，对基因操纵哺乳动物疾病发生和进展的研究可能会为药物发现描绘新的分子靶点，并为药物功效筛选提供新的平台。结论使用基因操纵哺乳动物模拟人类疾病和治疗实现了分子医学的承诺：分子生物化学与“经典”生物学和生理学的融合。外科医生拥有跨越两个世界的独特技能，可以帮助他们在这个不断扩大的领域取得成功。
Objective Transgenic and knockout technologies have emerged from the" molecular biology revolution" as unprecedented techniques for manipulating gene function in intact mice. The goals of this review are to outline the techniques of creating transgenic and knockout mice, and to demonstrate their use in elucidation of the molecular mechanisms underlying common surgical diseases.Summary Background Data Gain of gene function is created by transgenic technology, whereas gene function is ablated using gene knockouts. Each technique has distinctive applications and drawbacks. A unique feature of genetically manipulated mice is that combinatorial genetic experiments can be executed that precisely define the functional contribution of a gene to disease progression. Transgenic and knockout mouse models of wound healing, cardiovascular disease, transplant immunology, gut motility and inflammatory bowel disease, and oncology are beginning to illuminate the precise molecular regulation of these diseases. Transgenic technology has also been extended to larger mammals such as pigs, with the goal of using genetic manipulation of the xenogenic immune response to increase the availability of transplant organs. Continual refinements in gene manipulation technology in mice offer the opportunity to turn genes on or off at precise time intervals and in particular tissues, according to the needs of the investigator. Ultimately, investigation of disease development and progression in genetically manipulated mammals may delineate new molecular targets for drug discovery and provide novel platforms for drug efficacy screens.Conclusions Emulation of human disease and therapy using genetically manipulated mammals fulfills a promise of molecular medicine: fusion of molecular biochemistry with" classical" biology and physiology. Surgeons have unique skills spanning both worlds that can facilitate their success in this expanding arena.
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转基因小鼠中人载脂蛋白 C-III 的过度表达会导致载脂蛋白 B48 残余物的积累，并被过量的载脂蛋白 E 纠正。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
deSilva,HV;Lauer,SJ;Wang,J;Simonet,WS;Weisgraber,KH;Mahley,RW;Taylor,JM
通讯作者：
Taylor,JM
v-fos 和 v-rasHA 之间的合作在转基因表皮中诱导自主乳头状瘤，但不会恶性转化。
影响因子：
11.2
作者：
Greenhalgh,DA;Quintanilla,MI;Orengo,CC;Barber,JL;Eckhardt,JN;Rothnagel,JA;Roop,DR
通讯作者：
Roop,DR
DOI：
10.1161/01.atv.14.1.133
发表时间：
1994-01-01
期刊：
ARTERIOSCLEROSIS AND THROMBOSIS
影响因子：
--
作者：
NAKASHIMA, Y;PLUMP, AS;ROSS, R
通讯作者：
ROSS, R
影响因子：
15.9
作者：
ISHIBASHI, S;BROWN, MS;HERZ, J
通讯作者：
HERZ, J
影响因子：
56.9
作者：
WARDEN, CH;HEDRICK, CC;LUSIS, AJ
通讯作者：
LUSIS, AJ