C21 Fraction Refined from Marsdenia tenacissima-Induced Apoptosis is Enhanced by Suppression of Autophagy in Human Gastric Cell Lines.
C21 Fraction Refined from Marsdenia tenacissima-Induced Apoptosis is Enhanced by Suppression of Autophagy in Human Gastric Cell Lines.
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从 Marsdenia tenacissima 精制的 C21 组分通过抑制人胃细胞系中的自噬而增强诱导的细胞凋亡
DOI：
10.1021/acsomega.0c02748
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发表时间：
2020-10-06
期刊：
影响因子：
4.1
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Li K;Hao K;Zhang Y;Xu A;Wang Q;Du Y;Wu L;Chen B;Zhang W;Wang Z
C21甾体糖苷已被广泛报道用于治疗几种类型的癌症，并且广泛存在于通光藤中。本研究从M. tenacissima，并针对体外胃细胞系BGC-823、SGC-7901和AGS评估其抗癌效力。C21组份对胃癌细胞生长有明显的抑制作用，并使细胞周期阻滞。C21组分处理的胃细胞中的凋亡染色技术的结果证实了过量的活性氧的产生。C21组分还能提高SOD和H2 O2水平，尤其是与氯喹（CQ）合用时。C21组分的凋亡诱导潜力也通过蛋白质印迹法（western blot）上调促凋亡蛋白裂解的PARP和BAX以及下调抗凋亡蛋白Bcl-2和p-AKT来证明，特别是在存在自噬抑制剂CQ的情况下。结果表明，C21组分通过抑制细胞自噬，促进胃癌细胞凋亡。本研究结果为C21组分抗胃癌作用提供了新的机制。
C21 steroidal glycosides have been extensively reported for treating several types of cancer and are widely found in Marsdenia tenacissima. In this study, a C21 fraction was synthesized from M. tenacissima, and its anti-cancer potency was assessed against in vitro gastric cell lines BGC-823, SGC-7901, and AGS. Significant growth inhibition and cell cycle arrest were observed in C21 fraction-treated gastric cancer cells. The results of apoptotic staining techniques in C21 fraction-treated gastric cells were confirmed with excess reactive oxygen species generation. Moreover, SOD and H2O2 levels were increased by C21 fraction, especially when combined with chloroquine (CQ). The apoptotic inducing potential of C21 fraction was also evidenced by upregulation of proapoptotic proteins cleaved-PARP and BAX and downregulation of antiapoptotic proteins Bcl-2 and p-AKT by western blot, especially in the presence of the autophagy inhibitor CQ. The results showed that the apoptosis of gastric cancer cells caused by C21 fraction was enhanced by inhibiting autophagy. The current findings reveal a new mechanism for the antitumor activity of C21 fraction on gastric cancer.
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影响因子：
3.4
作者：
Chuang WL;Su CC;Lin PY;Lin CC;Chen YL
通讯作者：
Chen YL
影响因子：
9.7
作者：
Hu, Wei;Chen, Sang-Sang;Zhou, Hui-Jun
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Zhou, Hui-Jun
影响因子：
5.2
作者：
Wei F;Jiang X;Gao HY;Gao SH
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Gao SH
影响因子：
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作者：
Han SY;Ding HR;Zhao W;Teng F;Li PP
通讯作者：
Li PP
塞来昔布通过 PI3K/Akt 信号通路调节 SGC-7901 胃癌细胞凋亡和自噬
影响因子：
5.4
作者：
Liu M;Li CM;Chen ZF;Ji R;Guo QH;Li Q;Zhang HL;Zhou YN
通讯作者：
Zhou YN