Eukaryotic cells producing ribosomes deficient in Rpl1 are hypersensitive to defects in the ubiquitin-proteasome system.
Eukaryotic cells producing ribosomes deficient in Rpl1 are hypersensitive to defects in the ubiquitin-proteasome system.
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DOI：
10.1371/journal.pone.0023579
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Warner JR
中科院分区：
文献类型：
--
作者：
McIntosh KB;Bhattacharya A;Willis IM;Warner JR
最近已经很清楚，真核细胞中核糖体的错误装配可能产生的有害影响远远超出了核糖体的简单短缺。在这项工作中，我们发现，细胞缺乏核糖体蛋白L1（Rpl 1; Rpl 10a在哺乳动物）产生的核糖体缺乏Rpl 1输出到细胞质，并可以纳入多聚核糖体。这种有缺陷的核糖体的存在导致生长缓慢，并似乎使细胞对泛素-蛋白酶体系统中的病变高度敏感。几个基因，是合理的候选人的60 S亚基缺乏Rpl 1的降解未能做到这一点，这表明在核糖体亚基的监视的关键球员仍然有待发现有趣的是，尽管渲染细胞过敏的蛋白酶体抑制剂MG 132，Rpl 1的短缺部分抑制应激引起的暂时抑制MG 132引起的核糖体合成。
It has recently become clear that the misassembly of ribosomes in eukaryotic cells can have deleterious effects that go far beyond a simple shortage of ribosomes. In this work we find that cells deficient in ribosomal protein L1 (Rpl1; Rpl10a in mammals) produce ribosomes lacking Rpl1 that are exported to the cytoplasm and that can be incorporated into polyribosomes. The presence of such defective ribosomes leads to slow growth and appears to render the cells hypersensitive to lesions in the ubiquitin-proteasome system. Several genes that were reasonable candidates for degradation of 60S subunits lacking Rpl1 fail to do so, suggesting that key players in the surveillance of ribosomal subunits remain to be found. Interestingly, in spite of rendering the cells hypersensitive to the proteasome inhibitor MG132, shortage of Rpl1 partially suppresses the stress-invoked temporary repression of ribosome synthesis caused by MG132.
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影响因子：
9.2
作者：
Lam, Yun Wah;Lamond, Angus I.;Mann, Matthias;Andersen, Jens S.
通讯作者：
Andersen, Jens S.
影响因子：
10.5
作者：
Fujii, Kotaro;Kitabatake, Makoto;Ohno, Mutsuhito
通讯作者：
Ohno, Mutsuhito
影响因子：
4.8
作者：
JOHNSON, ES;MA, PCM;VARSHAVSKY, A
通讯作者：
VARSHAVSKY, A
影响因子：
16
作者：
Boulon S;Westman BJ;Hutten S;Boisvert FM;Lamond AI
通讯作者：
Lamond AI
影响因子：
16
作者：
Ferreira-Cerca, S;Pöll, G;Milkereit, P
通讯作者：
Milkereit, P