Comparative proteomic analysis of transition of saccharomyces cerevisiae from glucose-deficient medium to glucose-rich medium.
Comparative proteomic analysis of transition of saccharomyces cerevisiae from glucose-deficient medium to glucose-rich medium.
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DOI：
10.1186/1477-5956-10-40
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发表时间：
2012-06-12
期刊：
影响因子：
2
通讯作者：
Chiang HL
中科院分区：
文献类型：
--
作者：
Giardina BJ;Stanley BA;Chiang HL
当葡萄糖被添加到在不可发酵碳源中生长的酿酒酵母中时，编码核糖体、细胞周期和糖酵解蛋白的基因被诱导。与此相反，参与线粒体功能、线粒体异生和其他碳源利用的基因受到抑制。葡萄糖还引起质膜ATP酶的激活和促细胞生成酶和线粒体酶的失活。本研究的目的是使用iTRAQ标记质谱技术来鉴定其相对水平响应于葡萄糖再喂养而变化的蛋白质，并将蛋白质丰度的变化与转录和酶活性的变化相关联。我们使用了一种实验条件，当葡萄糖饥饿的细胞补充葡萄糖时，该条件会导致产酶酶的降解。这些酶被葡萄糖下调的鉴定作为内部对照。此外，我们试图确定新的蛋白质，无论是上调或下调葡萄糖。我们已经鉴定了新的和已知的蛋白质，它们在从含有低葡萄糖的培养基转移到含有高葡萄糖的培养基的细胞中改变它们的相对水平。上调的蛋白质包括核糖体亚基、参与蛋白质翻译的蛋白质和质膜ATP酶。下调的蛋白质包括小分子热休克蛋白、线粒体蛋白、糖酵解酶和促凋亡酶。Ach1p参与乙酸代谢，也受葡萄糖下调。我们已经确定了已知的蛋白质，以前已被报道，以及新的葡萄糖调节蛋白质的葡萄糖调节。核糖体蛋白和参与翻译的蛋白质的上调可导致蛋白质合成和营养摄取的增加。糖酵解酶、糖原合成酶和线粒体蛋白的下调可能导致糖酵解、糖原合成和线粒体功能的变化。这些变化可能有利于葡萄糖饥饿的细胞适应葡萄糖的添加。
When glucose is added to Saccharomyces cerevisiae grown in non-fermentable carbon sources, genes encoding ribosomal, cell-cycle, and glycolytic proteins are induced. By contrast, genes involved in mitochondrial functions, gluconeogenesis, and the utilization of other carbon sources are repressed. Glucose also causes the activation of the plasma membrane ATPase and the inactivation of gluconeogenic enzymes and mitochondrial enzymes. The goals of this study were to use the iTRAQ-labeling mass spectrometry technique to identify proteins whose relative levels change in response to glucose re-feeding and to correlate changes in protein abundance with changes in transcription and enzymatic activities. We used an experimental condition that causes the degradation of gluconeogenic enzymes when glucose starved cells are replenished with glucose. Identification of these enzymes as being down-regulated by glucose served as an internal control. Furthermore, we sought to identify new proteins that were either up-regulated or down-regulated by glucose. We have identified new and known proteins that change their relative levels in cells that were transferred from medium containing low glucose to medium containing high glucose. Up-regulated proteins included ribosomal subunits, proteins involved in protein translation, and the plasma membrane ATPase. Down-regulated proteins included small heat shock proteins, mitochondrial proteins, glycolytic enzymes, and gluconeogenic enzymes. Ach1p is involved in acetate metabolism and is also down-regulated by glucose. We have identified known proteins that have previously been reported to be regulated by glucose as well as new glucose-regulated proteins. Up-regulation of ribosomal proteins and proteins involved in translation may lead to an increase in protein synthesis and in nutrient uptake. Down-regulation of glycolytic enzymes, gluconeogenic enzymes, and mitochondrial proteins may result in changes in glycolysis, gluconeogenesis, and mitochondrial functions. These changes may be beneficial for glucose-starved cells to adapt to the addition of glucose.
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将果糖-1, 6-双磷酸酶导入 Vid 囊泡需要热休克蛋白 Ssa2p。
DOI：
10.1083/jcb.150.1.65
发表时间：
2000-07-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Brown CR;McCann JA;Chiang HL
通讯作者：
Chiang HL
影响因子：
4.8
作者：
Bond, Sarah;Forgac, Michael
通讯作者：
Forgac, Michael
DOI：
10.1099/00221287-138-8-1579
发表时间：
1992-08-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
BRANDAO, RL;CASTRO, IM;THEVELEIN, JM
通讯作者：
THEVELEIN, JM
影响因子：
4.8
作者：
Brown, C. Randell;Wolfe, Allison B.;Chiang, Hui-Ling
通讯作者：
Chiang, Hui-Ling
DOI：
10.1111/j.1432-1033.1992.tb16720.x
发表时间：
1992-03-15
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
FERNANDEZ, E;MORENO, F;RODICIO, R
通讯作者：
RODICIO, R