Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70 null flies.
Inducible and constitutive heat shock gene expression responds to modification of Hsp70 copy number in Drosophila melanogaster but does not compensate for loss of thermotolerance in Hsp70 null flies.
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DOI：
10.1186/1741-7007-6-5
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发表时间：
2008-01-22
期刊：
影响因子：
5.4
通讯作者：
Drohan, Brian W.
中科院分区：
文献类型：
--
作者：
Bettencourt, Brian R.;Hogan, Catherine C.;Nimali, Mario;Drohan, Brian W.
热休克蛋白Hsp70促进诱导的耐热性，在几乎每一个有机体检查日期。热休克蛋白70与其他应激反应蛋白的网络相互作用，解剖这些相互作用在引起热耐受性中的相对作用仍然很困难。在这里，我们研究热休克蛋白70基因拷贝数修饰的耐热性和多个应激反应基因在果蝇的表达的影响，以确定哪些基因可能代表应激耐受性的机制独立于热休克蛋白70。4株菌的Hsp70拷贝数与Hsp70表达和诱导的严重热休克耐受性呈正相关。当在仔细选择的温度下测定时，Hsp70无效苍蝇几乎完全缺乏耐热性。与预期相反，热预处理诱导的Hsp70表达水平的增加与菌株中其他7种诱导型Hsps水平的增加相关。此外，完全热休克蛋白70的损失导致上调的诱导热休克蛋白和六个组成性应激反应基因后，严重的热休克。热休克蛋白70拷贝数的修改定量和定性地影响多个其他应激反应基因的表达。热预处理后热休克蛋白70和其他热休克蛋白的绝对表达水平之间的正相关性表明新的调节机制。严重的热休克诱导新的基因表达模式和几乎总死亡率在Hsp70空菌株：在这种菌株中的基因表达的改变并不能弥补Hsp70的损失，但建议候选人过表达的研究。
The heat shock protein Hsp70 promotes inducible thermotolerance in nearly every organism examined to date. Hsp70 interacts with a network of other stress-response proteins, and dissecting the relative roles of these interactions in causing thermotolerance remains difficult. Here we examine the effect of Hsp70 gene copy number modification on thermotolerance and the expression of multiple stress-response genes in Drosophila melanogaster, to determine which genes may represent mechanisms of stress tolerance independent of Hsp70. Hsp70 copy number in four strains is positively associated with Hsp70 expression and inducible thermotolerance of severe heat shock. When assayed at carefully chosen temperatures, Hsp70 null flies are almost entirely deficient in thermotolerance. In contrast to expectations, increasing Hsp70 expression levels induced by thermal pretreatment are associated with increasing levels of seven other inducible Hsps across strains. In addition, complete Hsp70 loss causes upregulation of the inducible Hsps and six constitutive stress-response genes following severe heat shocks. Modification of Hsp70 copy number quantitatively and qualitatively affects the expression of multiple other stress-response genes. A positive association between absolute expression levels of Hsp70 and other Hsps after thermal pretreatment suggests novel regulatory mechanisms. Severe heat shocks induce both novel gene expression patterns and almost total mortality in the Hsp70 null strain: alteration of gene expression in this strain does not compensate for Hsp70 loss but suggests candidates for overexpression studies.
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影响因子：
3.3
作者：
Bettencourt, BR;Feder, ME;Cavicchi, S
通讯作者：
Cavicchi, S
影响因子：
64.8
作者：
Lee, D;Lis, JT
通讯作者：
Lis, JT
DOI：
10.1159/000468754
发表时间：
1990-01-01
期刊：
ENZYME
影响因子：
--
作者：
LINDQUIST, S;PETERSEN, R
通讯作者：
PETERSEN, R
影响因子：
8
作者：
Mayer, MP;Bukau, B
通讯作者：
Bukau, B
影响因子：
56.9
作者：
Auluck, PK;Chan, HYE;Bonini, NM
通讯作者：
Bonini, NM