The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
The role of melanocortin 1 receptor on melanogenesis pathway in skin color differentiation of red tilapia
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黑皮质素1受体对黑素生成途径在红罗非鱼肤色分化中的作用
DOI：
10.1016/j.aqrep.2021.100946
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发表时间：
2022-02
影响因子：
3.7
通讯作者：
Zai-jie Dong
中科院分区：
文献类型：
--
作者：
Lan-mei Wang;Bing-jie Jiang;Wen-bin Zhu;Jian-jun Fu;Ming-kun Luo;Wei Liu;Zai-jie Dong
皮肤颜色的分化和变异是制约红罗非鱼商业养殖发展的主要问题。mc1基因在鱼类色素沉着中的具体功能和调控机制尚不清楚。本研究探讨了红罗非鱼MSH -mc1基因序列特征、表达谱、定位及MSH -mc1在黑素形成通路中的作用。红罗非鱼的themc1rrf为1056 bp，对应351个氨基酸，属于g蛋白偶联受体家族。实时荧光定量PCR结果显示，mc1rmrna主要表达于PB（粉红色，散点黑点）鱼的脑组织和背侧皮肤组织。在红罗非鱼的早期发育阶段发现了Themc1rgene的表达。在孵化后48h （48hph）、24hph和卵裂期，Themc1rmRNA的表达量较高。免疫荧光分析显示Mc1r主要集中在皮肤表皮的细胞质和细胞间物质中。尾静脉注射2.5 mg g - 1的β -MSH可显著提高红罗非鱼背部和腹部皮肤的酪氨酸酶活性和黑色素水平。注射β -MSH后，红罗非鱼皮肤中mc1randtyrmrna的表达增加。这些结果表明，mc1通过与α-MSH结合，在鱼类黑色素形成途径中发挥重要作用。
The skin color differentiation and variation are the main problems limiting the development of red tilapia commercial culture. The specific function and regulation mechanism ofmc1rgene in fish pigmentation are still poorly understood. In this study, we investigated themc1rsequence characteristics, expression profiles, localization and melanocortins (MSH)-mc1rrole on melanogenesis pathway of red tilapia. Themc1rORF of red tilapia is1056 bp, corresponding to 351 amino acid, which belongs to a family of G-protein-coupled receptors. Quantitative real-time PCR demonstrated thatmc1rmRNA was mainly expressed in the brain and dorsal skin tissues of PB (pink with scattered black spots) fish. Themc1rgene was found to be expressed during early development stages of red tilapia. Themc1rmRNA expressions were high in the 48 h post-hatching (48hph), 24hph and cleavage stages. Immunofluorescence analysis revealed that Mc1r was concentrated mainly in the cytoplasm and intercellular substance of skin epidermis. Injection with caudal vein of ɑ-MSH at 2.5 mg g−1resulted in significantly higher tyrosinase activity and melanin level in the dorsal and ventral skin of red tilapia. The expression ofmc1randtyrmRNA in the skin of red tilapia increased after ɑ-MSH injection. These results suggested thatmc1rplays an important role in fish melanogenesis pathway through binding to α-MSH.
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影响因子：
3.5
作者：
Selz, Yvonne;Braasch, Ingo;Volff, Jean-Nicolas
通讯作者：
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影响因子：
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