Induced transcriptional profiling of phenylpropanoid pathway genes increased flavonoid and lignin content in Arabidopsis leaves in response to microbial products.
Induced transcriptional profiling of phenylpropanoid pathway genes increased flavonoid and lignin content in Arabidopsis leaves in response to microbial products.
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DOI：
10.1186/1471-2229-14-84
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发表时间：
2014-04-01
影响因子：
5.3
通讯作者：
McNear DH Jr
中科院分区：
文献类型：
--
作者：
Ali MB;McNear DH Jr
生物来源的土壤添加剂的生产和使用是肥料工业中增长最快的部门之一。这些产品已被证明可以提高作物产量，同时减少农田的肥料投入和养分损失。驱动初级生产力和土壤过程变化的机制尚不清楚，而与微生物产物使用相关的次级生产力变化则知之甚少。在这里，我们通过监测拟南芥中苯丙素（PP）途径的变化来研究对生物来源的土壤添加剂的次生代谢反应。本研究旨在测试其中一种产品（Soil Builder™-AF， SB）在不同时间施用后对次生代谢的影响。与多次施用相同产品相比，一次施用SB可增加拟南芥中黄酮类化合物的积累。用质谱法鉴定了黄酮醇和花青素等14种酚类化合物。主要化合物山奈酚-3,7- o -双-α- l-鼠李糖苷和槲皮素3,7-鼠李糖苷在TI处理下分别比对照增加3倍和4倍。其中花青素含量最高的是花青素3-鼠李糖苷（cyanidin 3-rhamnoglucoside），其含量分别比对照和TII增加了3倍和2倍。同时，PP通路关键酶（苯丙氨酸解氨酶、肉桂酸4-羟化酶、查尔酮合成酶、类黄酮-3′- o -羟化酶、黄酮醇合成酶1和二氢黄酮-4-还原酶）和调控基因（花青素色素生成2、MYB12、MYB113、MYB114、EGL3和TT8）的表达在两种处理（TI和TII）中均有所上调。此外，TI和TII诱导木质素途径基因（羟基肉桂酰基转移酶、咖啡因-辅酶a o -甲基转移酶、肉桂醇脱氢酶、肉桂酰基辅酶a还原酶、次级壁相关NAC结构域蛋白1、MYB58和MYB63）的表达，导致木质素含量积累高于对照。这些结果表明，添加微生物基土壤添加剂对苯丙素途径基因调控及其次生代谢产物的产生有明显的影响。这些发现为研究微生物基土壤添加剂的作用方式开辟了一条研究途径，这可能有助于食品、饲料、燃料和纤维的可持续生产。
The production and use of biologically derived soil additives is one of the fastest growing sectors of the fertilizer industry. These products have been shown to improve crop yields while at the same time reducing fertilizer inputs to and nutrient loss from cropland. The mechanisms driving the changes in primary productivity and soil processes are poorly understood and little is known about changes in secondary productivity associated with the use of microbial products. Here we investigate secondary metabolic responses to a biologically derived soil additive by monitoring changes in the phenlypropanoid (PP) pathway in Arabidopsis thaliana. This study was designed to test the influence of one of these products (Soil Builder™-AF, SB) on secondary metabolism after being applied at different times. One time (TI) application of SB to Arabidopsis increased the accumulation of flavonoids compared to multiple (TII) applications of the same products. Fourteen phenolic compounds including flavonols and anothocyanins were identified by mass spectrometry. Kaempferol-3,7-O-bis-α-L-rhamnoside and quercetin 3,7-dirhamnoside, the major compounds, increased 3-fold and 4-fold, respectively compared to control in the TI treatment. The most abundant anthocyanin was cyanidin 3-rhamnoglucoside, which increased 3-fold and 2-fold in TI compared to the control and TII, respectively. Simultaneously, the expression of genes coding for key enzymes in the PP pathway (phenylalanine ammonia lyase, cinnamate 4-hydroxylase, chalcone synthase, flavonoid-3′-O-hydroxylase, flavonol synthase1 and dihydroflavonol-4-reductase) and regulatory genes (production of anthocyanin pigment2, MYB12, MYB113, MYB114, EGL3, and TT8) were up-regulated in both treatments (TI and TII). Furthermore, application of TI and TII induced expression of the lignin pathway genes (hydroxyl cinamyl transferase, caffeyl-CoA O-methyl transferase, cinnamyl alcohol dehydrogenase, cinnamyl-CoA reductase, secondary wall-associated NAC domain protein1, MYB58 and MYB63 resulting in higher accumulation of lignin content compared to the control. These results indicate that the additions of microbially based soil additives have a perceptible influence on phenylpropanoid pathway gene regulation and its production of secondary metabolites. These findings open an avenue of research to investigate the mode of action of microbially-based soil additives which may assist in the sustainable production of food, feed, fuel and fiber.
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影响因子：
3.6
作者：
Adesemoye, A. O.;Torbert, H. A.;Kloepper, J. W.
通讯作者：
Kloepper, J. W.
影响因子：
7.4
作者：
Deluc, L;Barrieu, F;Hamdi, S
通讯作者：
Hamdi, S
影响因子：
7.4
作者：
Huang, Junli;Gu, Min;Chen, Zhixiang
通讯作者：
Chen, Zhixiang
影响因子：
5
作者：
Garcia-Mediavilla, Victoria;Crespo, Irene;Gonzalez-Gallego, Javier
通讯作者：
Gonzalez-Gallego, Javier
影响因子：
7.4
作者：
Bhargava, Apurva;Mansfield, Shawn D.;Ellis, Brian E.
通讯作者：
Ellis, Brian E.