Enhanced osseointegration of titanium implant through the local delivery of transcription factor SATB2.
Enhanced osseointegration of titanium implant through the local delivery of transcription factor SATB2.
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DOI：
10.1016/j.biomaterials.2011.07.072
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发表时间：
2011-11
期刊：
影响因子：
14
通讯作者：
Chen, J.
中科院分区：
文献类型：
--
作者：
Yan, S. G.;Zhang, J.;Tu, Q. S.;Ye, J. H.;Luo, E.;Schuler, M.;Kim, M. S.;Griffin, T.;Zhao, J.;Duan, X. J.;Cochran, D. J.;Murray, D.;Yang, P. S.;Chen, J.
钛植入物广泛用于牙科和骨科手术。然而，植入后，种植体周围的骨再生是一个相对缓慢的过程。本研究评估了SATB 2是否可以增强钛种植体的骨整合。为了确定SATB 2在种植体整合中的作用，在我们建立的转基因TVA小鼠中，在钛种植体放置之前，将编码SATB 2的两种不同病毒（PBABE-Satb 2病毒或RCAS-Satb 2病毒）局部施用到骨缺损。植入后7天和21天，分离股骨用于定量实时RT-PCR、H&E染色、免疫组织化学（IHC）染色和显微计算机断层扫描（microCT）分析。定量实时RT-PCR结果表明，在体内过表达SATB 2增强表达水平的有效的成骨转录因子和骨基质蛋白。我们还发现，植入后21天，RCAS-Satb 2组和RCAS组之间SATB 2、Osx、Runx 2、COLI、OC和BSP的表达水平没有显著差异。组织学分析显示，SATB 2过表达显著增强了植入后的新骨形成和骨与植入物的接触。免疫组化染色分析显示，SATB 2的强制表达增加了植入物周围BSP阳性细胞的数量。MicroCT分析表明，体内过表达SATB 2显著增加了种植体周围新形成骨的密度。这些结果表明，在体内过表达的SATB 2显着加速钛种植体的骨整合和SATB 2可以作为一个有效的分子，在促进组织再生。
Titanium implants are widely used in dentistry and orthopaedic surgery. Nevertheless, bone regeneration around the implant is a relatively slow process, after placement. This study assessed whether SATB2 can enhance osseointegration of a titanium implant. To determine the effect of SATB2 in implant integration, two different viruses encoding SATB2 (PBABE-Satb2 virus or RCAS-Satb2 virus) were locally administered to the bone defect prior to titanium implant placement in our established transgenic TVA mice. Seven and 21 days post implantation, the femurs were isolated for quantitative real-time RT-PCR, H&E staining, immunohistochemical (IHC) staining, and microcomputed tomography (microCT) analysis. Quantitative real-time RT-PCR results demonstrated that the in vivo overexpression of SATB2 enhanced expression levels of potent osteogenic transcription factors and bone matrix proteins. We also found that 21 days after implantation, there were no significant differences in the expression levels of SATB2, Osx, Runx2, COLI, OC, and BSP between the RCAS-Satb2 group and the RCAS group. Histological analysis showed that SATB2 overexpression significantly enhanced new bone formation and bone-to-implant contact after implantation. IHC staining analysis revealed that forced expression of SATB2 increased the number of BSP-positive cells surrounding the implant. MicroCT analysis demonstrated that in vivo overexpression of SATB2 significantly increased the density of the newly formed bone surrounding the implant. These results conclude that in vivo overexpression of SATB2 significantly accelerates osseointegration of titanium implants and SATB2 can serve as a potent molecule in promoting tissue regeneration.
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