The Distinct Effects of Estrogen and Hydrostatic Pressure on Mesenchymal Stem Cells Differentiation: Involvement of Estrogen Receptor Signaling
The Distinct Effects of Estrogen and Hydrostatic Pressure on Mesenchymal Stem Cells Differentiation: Involvement of Estrogen Receptor Signaling
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雌激素和静水压对间充质干细胞分化的独特影响：雌激素受体信号传导的参与
DOI：
10.1007/s10439-016-1631-5
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发表时间：
2016-06
影响因子：
3.8
通讯作者：
Zhang Min
中科院分区：
文献类型：
--
作者：
Zhao Ying;Yi Fei-Zhou;Zhao Yin-Hua;Chen Yong-Jin;Ma Heng;Zhang Min
This study aimed to investigate the differential and synergistic effects of mechanical stimulation and estrogen on the proliferation and osteogenic or chondrogenic differentiation potential of bone marrow mesenchymal stem cells (BMSCs) and the roles of estrogen receptor (ER) in them. BMSCs were isolated and cultured using the whole bone marrow adherence method, and flow cytometry was used to identify the surface marker molecules of BMSCs. Cells were pre-treated with 1 nM 17β-estradiol or 1 nM of the estrogen receptor antagonist tamoxifen. Then, the cells were stimulated with hydrostatic pressure. Assessment included flow cytometry analysis of the cell cycle; immunofluorescent staining for F-actin; protein quantification for MAPK protein; and mRNA analysis for Col I, OCN, OPN and BSP after osteogenic induction and Sox-9, Aggrecan and Col-II after chondrogenic induction. Hydrostatic pressure (90 kPa/1 h) and 1 nM 17β-estradiol enhanced the cellular proliferation ability and the cytoskeleton activity but without synergistic biological effects. Estrogen activated ERKs and JNKs simultaneously and promoted the osteogenic differentiation, whereas the pressure just caused JNK-1/2 activation and promoted the chondrogenic differentiation of BMSCs. Estrogen had antagonism effect on chondrogenic promotion of hydrostatic pressure. Mechanobiological effects of hydrostatic pressure are closely associated with ERα activity. MAPK molecules and F-actin were likely to be important mediator molecules in the ER-mediated mechanotransduction of BMSCs.
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影响因子：
4
作者：
Liao, Qing-Chuan;Li, Ya-Lin;Qin, Yan-Fang;Quarles, L. Darryl;Xu, Kang-Kang;Li, Rong;Zhou, Hong-Hao;Xiao, Zhou-Sheng
通讯作者：
Xiao, Zhou-Sheng
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ
影响因子：
5.2
作者：
Huang, CYC;Reuben, PM;Cheung, HS
通讯作者：
Cheung, HS
DOI：
--
发表时间：
2009-02
期刊：
Journal of the South Carolina Medical Association
影响因子：
--
作者：
E. Farrar;H. Mitchell
通讯作者：
E. Farrar;H. Mitchell
影响因子：
2
作者：
Kee Cc
通讯作者：
Kee Cc