Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells.
Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells.
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在胚胎发育过程中，内皮细胞（ECs）经历血管发生，形成一个原始神经丛，并组装成由壁细胞稳定的血管组成的网络，这些血管具有分子上不同的动脉和静脉特征。这种有组织的脉管系统是在血流开始之前建立的，依赖于一系列复杂的信号事件，这些事件主要在动物模型中阐明，但在人类中研究和了解较少。在这里，我们开发了一种简单的人类多能干细胞血管分化方案，可以同时生成内皮细胞、周细胞和平滑肌细胞。当该方案应用于3D水凝胶时，我们证明它概括了早期人类血管形成的动态过程，包括获得不同的动脉和静脉命运，导致血管生成血管生成模型丛（VAMP）。VAMP捕获了血管发生、血管生成和血管网络形成的主要阶段，是一种简单、快速、可扩展的模型系统，用于体外研究早期人类血管发育。
During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro.
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影响因子：
3.7
作者：
Lee SJ;Kim KH;Yoon YS
通讯作者：
Yoon YS
影响因子：
11.8
作者：
Corada M;Nyqvist D;Orsenigo F;Caprini A;Giampietro C;Taketo MM;Iruela-Arispe ML;Adams RH;Dejana E
通讯作者：
Dejana E
影响因子：
4
作者：
Kano, MR;Morishita, Y;Miyazawa, K
通讯作者：
Miyazawa, K
影响因子：
5.9
作者：
Faal, Tannaz;Phan, Duc T. T.;Inlay, Matthew A.
通讯作者：
Inlay, Matthew A.
影响因子：
20.3
作者：
Aranguren, Xabier L.;Agirre, Xabier;Luttun, Aernout
通讯作者：
Luttun, Aernout