Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of Saccharomyces pastorianus from Dekkera spp.
Application of Micropore Device for Accurate, Easy, and Rapid Discrimination of Saccharomyces pastorianus from Dekkera spp.
复制标题
用于从Dekkera spp的糖疗法的准确，易于辨别的微孔装置。
DOI：
10.3390/bios11080272
复制
发表时间：
2021-08-12
期刊：
影响因子：
--
通讯作者：
Kataoka M
中科院分区：
文献类型：
--
作者：
Yokota K;Takeo A;Abe H;Kurokawa Y;Hashimoto M;Kajimoto K;Tanaka M;Murayama S;Nakajima Y;Taniguchi M;Kataoka M
可追溯性分析，例如用于发酵的酵母的识别和区分，对于确保酿造过程中的生产效率和产品安全非常重要。然而，基于形态和生理特性的常规方法存在耗时、灵敏度低等缺点。在本研究中，采用电阻脉冲法（RPM）通过测量流经微米级孔的细胞的离子电流响应来区分巴斯德酵母和异常德克酵母以及巴斯德酵母和布鲁塞尔酵母。脉冲信号的高度和形状用于同时测量单个细胞的尺寸、形状和表面电荷。准确区分巴斯德酵母和德克酵母。观察到召回率为 96.3 ± 0.8%。此外，通过评估当前离子封锁的波形形状来定量检测出芽的巴斯德酵母。我们展示了用于检测 Dekkera spp 污染的 RPM 的概念验证演示。在巴斯德酵母中，通过定量检测出芽细胞来监测巴斯德酵母的发酵情况。
Traceability analysis, such as identification and discrimination of yeasts used for fermentation, is important for ensuring manufacturing efficiency and product safety during brewing. However, conventional methods based on morphological and physiological properties have disadvantages such as time consumption and low sensitivity. In this study, the resistive pulse method (RPM) was employed to discriminate between Saccharomyces pastorianus and Dekkera anomala and S. pastorianus and D. bruxellensis by measuring the ionic current response of cells flowing through a microsized pore. The height and shape of the pulse signal were used for the simultaneous measurement of the size, shape, and surface charge of individual cells. Accurate discrimination of S. pastorianus from Dekkera spp. was observed with a recall rate of 96.3 ± 0.8%. Furthermore, budding S. pastorianus was quantitatively detected by evaluating the shape of the waveform of the current ionic blockade. We showed a proof-of-concept demonstration of RPM for the detection of contamination of Dekkera spp. in S. pastorianus and for monitoring the fermentation of S. pastorianus through the quantitative detection of budding cells.
登录
查看更多内容
影响因子：
3.7
作者：
Fischer, Dennis;Buchbinder, Joern H.;Flassig, Robert J.
通讯作者：
Flassig, Robert J.
影响因子：
3
作者：
NG, B;BARRY, PH
通讯作者：
BARRY, PH
影响因子：
4.6
作者：
Tsutsui, Makusu;Yoshida, Takeshi;Kawai, Tomoji
通讯作者：
Kawai, Tomoji
影响因子：
4
作者：
Kopecka, J.;Nemec, M.;Matoulkova, D.
通讯作者：
Matoulkova, D.
访问电阻对纳米粒子通过纳米孔移位引起的电阻脉冲的影响
影响因子：
3.9
作者：
Wang, Junrong;Ma, Jian;Hu, Guoqing
通讯作者：
Hu, Guoqing