Separation of dansylated 17β-estradiol, 17α-estradiol, and estrone on a single HPLC column for simultaneous quantitation by LC-MS/MS.
Separation of dansylated 17β-estradiol, 17α-estradiol, and estrone on a single HPLC column for simultaneous quantitation by LC-MS/MS.
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DOI：
10.1007/s00216-013-6710-y
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发表时间：
2013-04
影响因子：
4.3
通讯作者：
Prokai-Tatrai, Katalin
中科院分区：
文献类型：
--
作者：
Szarka, Szabolcs;Vien Nguyen;Prokai, Laszlo;Prokai-Tatrai, Katalin
We show here that baseline separation of dansylated estrone, 17β-estradiol and 17α-estradiol can be done, contrary to previous reports, within a short run time on a single RP-LC analytical column packed with particles bonded with phenyl-hexyl stationary phase. The chromatographic method coupled with isotope-dilution tandem MS offers a simple assay enabling the simultaneous analysis of these analytes. The method employs 13C-labeled estrogens as internal standards to eliminate potential matrix effects arising from the use of deuterated estrogens. The assay also offers adequate accuracy and sensitivity to be useful for biological samples. The practical applicability of the validated method is demonstrated by the quantitative analyses of in vivo samples obtained from rats treated with Premarin®.
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影响因子：
1.7
作者：
Prokai, Laszlo;Frycak, Petr;Nguyen, Vien
通讯作者：
Nguyen, Vien
影响因子：
1.7
作者：
Prokai-Tatrai, Katalin;Bonds, Darius;Prokai, Laszlo
通讯作者：
Prokai, Laszlo
影响因子：
2.7
作者：
Blair, Ian A.
通讯作者：
Blair, Ian A.
DOI：
10.1016/j.jsbmb.2011.07.009
发表时间：
2011-11-01
影响因子：
4.1
作者：
Ayan, Diana;Roy, Jenny;Poirier, Donald
通讯作者：
Poirier, Donald
影响因子：
2.7
作者：
Hobe, G;Schön, R;Zimmermann, H
通讯作者：
Zimmermann, H