Expression of serum amyloid A in uterine cervical cancer.
Expression of serum amyloid A in uterine cervical cancer.
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子宫宫颈癌中血清淀粉样蛋白A的表达。
DOI：
10.1186/1746-1596-9-16
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发表时间：
2014-01-21
影响因子：
2.6
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Ren Y;Wang H;Lu D;Xie X;Chen X;Peng J;Hu Q;Shi G;Liu S
血清淀粉样蛋白A（SAA）是一种急性时相蛋白，主要在肝脏中表达。然而，最近也发现其在肝外组织，特别是肿瘤组织中的表达。在我们的研究中，我们调查了SAA在子宫颈癌中的表达，我们的结果表明其作为血清生物标志物的潜力。应用实时定量聚合酶链反应（RT-PCR）、免疫组化（IHC）和酶联免疫吸附试验（ELISA）检测非肿瘤性病变（NNL）、宫颈上皮内瘤样病变（CIN）和宫颈癌（CC）患者组织和血清中SAA基因和蛋白的表达水平。与NNL相比，SAA基因（SAA 1和SAA 4）在子宫CC中的表达水平显著更高（平均拷贝数分别为：138.7 vs.5.01，P < 0.000; 1.8 vs.0.079，P = 0.001）。免疫组化结果显示，宫颈腺癌和鳞状细胞癌组织中SAA蛋白质呈胞浆染色。SAA的中位血清浓度（μg/ml）在NNL患者中为6.02，在CIN患者中为10.98（P = 0.31）。相反，子宫CC患者中位血清SAA浓度为23.7 μg/ml，显著高于NNL组（P = 0.002）和CIN组（P = 0.024）的SAA浓度。我们的数据表明SAA可能是子宫CC细胞的产物。CC患者血清中的高SAA浓度可能在监测疾病发生方面发挥作用，并可能具有治疗应用。本文的虚拟幻灯片可以在这里找到：http://www.diagnosticpathology.diagnomx.eu/vs/1433263219102962。
As an acute-phase protein, serum amyloid A (SAA) is expressed primarily in the liver. However, its expression in extrahepatic tissues, especially in tumor tissues, was also demonstrated recently. In our study, we investigated the expression of SAA in uterine cervical carcinomas, and our results suggested its potential as a serum biomarker. Quantitative real-time polymerase chain reaction (RT-PCR), immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA) were used to evaluate the SAA gene and protein expression levels in the tissues and sera of patients with non-neoplastic lesions (NNLs), cervical intraepithelial neoplasia (CIN) and cervical carcinoma (CC). Compared with NNLs, the SAA gene (SAA1 and SAA4) expression levels were significantly higher in uterine CC (mean copy numbers: 138.7 vs. 5.01, P < 0.000; and 1.8 vs. 0.079, P = 0.001, respectively) by real-time PCR. IHC revealed cytoplasmic SAA protein staining in tissues from adenocarcinoma and squamous cell carcinoma of the cervix. The median serum concentrations (μg/ml) of SAA were 6.02 in patients with NNLs and 10.98 in patients with CIN (P = 0.31). In contrast, the median serum SAA concentration was 23.7 μg/ml in uterine CC patients, which was significantly higher than the SAA concentrations of the NNL group (P = 0.002) and the CIN group (P = 0.024). Our data suggested that SAA might be a uterine CC cell product. High SAA concentrations in the serum of CC patients may have a role in monitoring disease occurrence and could have therapeutic applications. The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1433263219102962.
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影响因子：
4.4
作者：
Connolly, Mary;Marrelli, Alessandra;Fearon, Ursula
通讯作者：
Fearon, Ursula
DOI：
10.1016/j.bbrc.2005.03.069
发表时间：
2005-05-13
影响因子：
3.1
作者：
Lee, HY;Kim, MK;Bae, YS
通讯作者：
Bae, YS
DOI：
10.1016/j.bbrc.2003.12.096
发表时间：
2004-02-06
影响因子：
3.1
作者：
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通讯作者：
Yoshizaki, K
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影响因子：
2.6
作者：
Liu H;Liu S;Wang H;Xie X;Chen X;Zhang X;Zhang Y
通讯作者：
Zhang Y
影响因子：
--
作者：
Mullan, RH;Bresnihan, B;Fearon, U
通讯作者：
Fearon, U