Electrochemistry Combined with LC-HRMS: Elucidating Transformation Products of the Recalcitrant Pharmaceutical Compound Carbamazepine Generated by the White-Rot Fungus Pleurotus ostreatus.
Electrochemistry Combined with LC-HRMS: Elucidating Transformation Products of the Recalcitrant Pharmaceutical Compound Carbamazepine Generated by the White-Rot Fungus Pleurotus ostreatus.
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电化学与 LC-HRMS 相结合：阐明白腐真菌平菇产生的顽抗药物化合物卡马西平的转化产物
DOI：
10.1021/acs.est.5b02229
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发表时间：
2015
影响因子：
11.4
通讯作者：
Reemtsma T.
中科院分区：
文献类型：
--
作者：
Seiwert B;Hadar Y;Reemtsma T.
必须确定环境污染物的转化产物(TP)，以了解生物降解过程和反应机理，并评估处理过程的效率。电化学池(EC)氧化与LC-HRMS分析相结合的方法是一种快速测定和鉴定天然微生物过程产生的TPS的方法。将难治药物卡马西平(CBZ)的TPS用于白腐菌平菇(Pleurotus Ostreatus)TPS的靶向筛选。采用LC-HRMS的EC有助于TPS的检测和鉴定，因为产物光谱不与生物来源的代谢物重叠，并且可以使用较高的底物浓度。通过对分子离子的靶向筛选检测到微生物过程中形成的10个TP，根据特征碎片离子进行筛选检测到另外4个TP。其中三种TPS以前从未被报道过。对于CBZ，EC和LC-HRMS被发现在确定筛查目标方面比软件工具更有效，在TP识别方面比单独进行非靶点筛查更快。具有LC-HRMS的EC可用于向MS数据库提供大量环境污染物的可能TPS的光谱，以进行有效的目标筛选。
Transformation products (TPs) of environmental pollutants must be identified to understand biodegradation processes and reaction mechanisms and to assess the efficiency of treatment processes. The combination of oxidation by an electrochemical cell (EC) with analysis by liquid chromatography–high-resolution mass spectrometry (LC–HRMS) is a rapid approach for the determination and identification of TPs generated by natural microbial processes. Electrochemically generated TPs of the recalcitrant pharmaceutical carbamazepine (CBZ) were used for a target screening for TPs formed by the white-rot fungusPleurotus ostreatus. EC with LC–HRMS facilitates detection and identification of TPs because the product spectrum is not superimposed with biogenic metabolites and elevated substrate concentrations can be used. A group of 10 TPs formed in the microbial process were detected by target screening for molecular ions, and another 4 were detected by screening on the basis of characteristic fragment ions. Three of these TPs have never been reported before. For CBZ, EC with LC–HRMS was found to be more effective than software tools in defining targets for the screening and faster than nontarget screening alone in TP identification. EC with LC-HRMS may be used to feed MS databases with spectra of possible TPs of larger numbers of environmental contaminants for an efficient target screening.
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影响因子：
2
作者：
Jurva, U;Wikström, HV;Bruins, AP
通讯作者：
Bruins, AP
用过氧化氢和血红蛋白或硫酸亚铁替代混合功能氧化酶 N-去甲基化
影响因子：
3.7
作者：
S. Zbaida;D. Gilhar;J. Silman
通讯作者：
J. Silman
9-吖啶甲醛的水合平衡
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
R. McCLELLAND;P. Sukhai;K. Engell;P. E. Sørensen
通讯作者：
P. E. Sørensen
关于二苯并[b,f]氧杂并[d]氮杂衍生物的反应。
DOI：
--
发表时间：
1978
期刊：
影响因子：
--
作者：
K. Kawashima;T. Ishiguro
通讯作者：
T. Ishiguro