Early Signals of Vaccine-driven Perturbation Seen in Pneumococcal Carriage Population Genomic Data.
Early Signals of Vaccine-driven Perturbation Seen in Pneumococcal Carriage Population Genomic Data.
复制标题
DOI：
10.1093/cid/ciz404
复制
发表时间：
2020-03-17
期刊：
影响因子：
--
通讯作者：
Bentley SD
中科院分区：
文献类型：
--
作者：
Chaguza C;Heinsbroek E;Gladstone RA;Tafatatha T;Alaerts M;Peno C;Cornick JE;Musicha P;Bar-Zeev N;Kamng'ona A;Kadioglu A;McGee L;Hanage WP;Breiman RF;Heyderman RS;French N;Everett DB;Bentley SD
肺炎球菌结合疫苗（PCV）在全球范围内减少了肺炎球菌疾病。肺炎球菌基因组调查阐明PCV对人口结构的影响，但很少在低收入环境中进行，尽管疾病负担很高。我们进行了全基因组测序（WGS）的660个肺炎球菌分离株收集通过调查从健康的运营商2年13价PCV（PCV 13）的介绍和1年后推出在北方马拉维。我们调查了种群结构的变化，谱系内血清型动态，血清型多样性，抗生素耐药（ABR）和辅助基因的频率。在<5岁的儿童中，疫苗血清型（VT）的频率和多样性在PCV后显著下降，但在≥5岁的人群中无显著变化。VT血清型的清除在不同遗传背景（谱系）中是一致的。在<5岁的儿童中，非疫苗血清型（NVT）-即7 C，15 B/C和23 A-增加，但28 F在两个年龄组中均增加。虽然最近显示，由于替换血清型，PCV后的携带率保持稳定，但NVT的多样性没有变化。此外，中等青霉素耐药谱系的频率减少后PCV。虽然ABR基因的频率保持稳定，但其他辅助基因，特别是与移动的遗传因子和细菌素相关的基因，在PCV后出现频率变化。我们证明了PCV后显著的人群重组的证据，这是由疫苗血清型频率降低和主要在5岁以下儿童中的少数NVT频率增加所驱动的。WGS的持续监测对于充分了解PCV后残留VT和替代NVT血清型的动态仍然至关重要。在马拉维引入13价肺炎球菌结合疫苗（PCV 13）后，各谱系携带疫苗血清型的频率显著下降;血清型7 C、15 B/C、23 A和28 F增加;抗生素耐药率保持稳定;某些辅助基因的频率发生变化。
Pneumococcal conjugate vaccines (PCVs) have reduced pneumococcal diseases globally. Pneumococcal genomic surveys elucidate PCV effects on population structure but are rarely conducted in low-income settings despite the high disease burden. We undertook whole-genome sequencing (WGS) of 660 pneumococcal isolates collected through surveys from healthy carriers 2 years from 13-valent PCV (PCV13) introduction and 1 year after rollout in northern Malawi. We investigated changes in population structure, within-lineage serotype dynamics, serotype diversity, and frequency of antibiotic resistance (ABR) and accessory genes. In children <5 years of age, frequency and diversity of vaccine serotypes (VTs) decreased significantly post-PCV, but no significant changes occurred in persons ≥5 years of age. Clearance of VT serotypes was consistent across different genetic backgrounds (lineages). There was an increase of nonvaccine serotypes (NVTs)—namely 7C, 15B/C, and 23A—in children <5 years of age, but 28F increased in both age groups. While carriage rates have been recently shown to remain stable post-PCV due to replacement serotypes, there was no change in diversity of NVTs. Additionally, frequency of intermediate-penicillin-resistant lineages decreased post-PCV. Although frequency of ABR genes remained stable, other accessory genes, especially those associated with mobile genetic element and bacteriocins, showed changes in frequency post-PCV. We demonstrate evidence of significant population restructuring post-PCV driven by decreasing frequency of vaccine serotypes and increasing frequency of few NVTs mainly in children under 5. Continued surveillance with WGS remains crucial to fully understand dynamics of the residual VTs and replacement NVT serotypes post-PCV. After 13-valent pneumococcal conjugate vaccine (PCV13) introduction in Malawi, the frequency of carried vaccine serotypes decreased significantly across lineages; there was an increase of serotypes 7C, 15B/C, 23A, and 28F; antibiotic resistance rates remained stable; and certain accessory genes changed in frequency.
登录
查看更多内容
影响因子：
3.9
作者：
Argimón S;Abudahab K;Goater RJE;Fedosejev A;Bhai J;Glasner C;Feil EJ;Holden MTG;Yeats CA;Grundmann H;Spratt BG;Aanensen DM
通讯作者：
Aanensen DM
在马拉维共轭疫苗之前，种群遗传结构，抗生素抗性，胶囊切换和侵入性肺炎的演变。
影响因子：
5.5
作者：
Chaguza C;Cornick JE;Andam CP;Gladstone RA;Alaerts M;Musicha P;Peno C;Bar-Zeev N;Kamng'ona AW;Kiran AM;Msefula CL;McGee L;Breiman RF;Kadioglu A;French N;Heyderman RS;Hanage WP;Bentley SD;Everett DB
通讯作者：
Everett DB
DOI：
10.1099/00221287-144-11-3049
发表时间：
1998-11-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Enright, MC;Spratt, BG
通讯作者：
Spratt, BG
影响因子：
56.3
作者：
Mackenzie, Grant A.;Hill, Philip C.;Corrah, Tumani
通讯作者：
Corrah, Tumani
影响因子：
3.7
作者：
Everett, Dean B.;Cornick, Jennifer;Bentley, Stephen D.
通讯作者：
Bentley, Stephen D.